Reactivity of monoclonal antibodies to species-specific antigens of Entamoeba histolytica.

H. Tachibana, S. Kobayashi, K. Nagakura, Y. Kaneda, T. Takeuchi

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Abstract

Twenty monoclonal antibodies were produced against trophozoites of Entamoeba histolytica strains HK-9 and HM-1: IMSS. When reactivity to various enteric protozoa was examined by an indirect fluorescence antibody test, 15 of the monoclonal antibodies were strongly reactive with E. histolytica trophozoites. Species-specific antigens recognized by these monoclonal antibodies were located on the plasma membrane, nucleus, cytoplasm, and cytoskeletal structures of the trophozoites. Two of the remaining five monoclonals reacted strongly with trophozoites of the E. histolytica-like Laredo strain. The determinant antigen was located in the cytoplasm. The three remaining monoclonal antibodies were found to recognize cross-reactive antigens between E. histolytica and E. histolytica-like Laredo, E. hartmanni, E. coli, Dientamoeba fragilis, Giardia lamblia, and Trichomonas hominis. These three antibodies were also reactive with T. vaginalis and mammalian cells such as HeLa cells. Thus, the combined use of monoclonal antibodies seems capable of distinguishing E. histolytica and/or E. histolytica-like Laredo from other enteric protozoa.

Original languageEnglish
Pages (from-to)329-334
Number of pages6
JournalJournal of Protozoology
Volume38
Issue number4
Publication statusPublished - 1991 Jul

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Entamoeba histolytica
Monoclonal Antibodies
Trophozoites
Antigens
Dientamoeba
Cytoplasm
Trichomonas
Giardia lamblia
Antibodies
HeLa Cells
Fluorescence
Cell Membrane
Escherichia coli

ASJC Scopus subject areas

  • Parasitology

Cite this

Tachibana, H., Kobayashi, S., Nagakura, K., Kaneda, Y., & Takeuchi, T. (1991). Reactivity of monoclonal antibodies to species-specific antigens of Entamoeba histolytica. Journal of Protozoology, 38(4), 329-334.

Reactivity of monoclonal antibodies to species-specific antigens of Entamoeba histolytica. / Tachibana, H.; Kobayashi, S.; Nagakura, K.; Kaneda, Y.; Takeuchi, T.

In: Journal of Protozoology, Vol. 38, No. 4, 07.1991, p. 329-334.

Research output: Contribution to journalArticle

Tachibana, H, Kobayashi, S, Nagakura, K, Kaneda, Y & Takeuchi, T 1991, 'Reactivity of monoclonal antibodies to species-specific antigens of Entamoeba histolytica.', Journal of Protozoology, vol. 38, no. 4, pp. 329-334.
Tachibana H, Kobayashi S, Nagakura K, Kaneda Y, Takeuchi T. Reactivity of monoclonal antibodies to species-specific antigens of Entamoeba histolytica. Journal of Protozoology. 1991 Jul;38(4):329-334.
Tachibana, H. ; Kobayashi, S. ; Nagakura, K. ; Kaneda, Y. ; Takeuchi, T. / Reactivity of monoclonal antibodies to species-specific antigens of Entamoeba histolytica. In: Journal of Protozoology. 1991 ; Vol. 38, No. 4. pp. 329-334.
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