Twenty monoclonal antibodies were produced against trophozoites of Entamoeba histolytica strains HK‐9 and HM‐1: IMSS. When reactivity to various enteric protozoa was examined by an indirect fluorescence antibody test, 15 of the monoclonal antibodies were strongly reactive with E. histolytica trophozoites. Species‐specific antigens recognized by these monoclonal antibodies were located on the plasma membrane, nucleus, cytoplasm, and cytoskeletal structures of the trophozoites. Two of the remaining five monoclonals reacted strongly with trophozoites of the E. histolytica‐like Laredo strain. The determinant antigen was located in the cytoplasm. The three remaining monoclonal antibodies were found to recognize cross‐reactive antigens between E. histolytica and E. histolytica‐like Laredo, E. hartmanni, E. coli, Dientamoeba fragilis, Giardia lamblia, and Trichomonas hominis. These three antibodies were also reactive with T. vaginalis
|Number of pages||6|
|Journal||The Journal of Protozoology|
|Publication status||Published - 1991 Jul|
- Indirect fluorescence antibody test
- Western blotting.
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