Reactivity of Monoclonal Antibodies to Species‐Specific Antigens of Entamoeba histolytica

HIROSHI TACHIBANA, SEIKI KOBAYASHI, KOUICHI NAGAKURA, YOSHIMASA KANEDA, TSUTOMU TAKEUCHI

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Abstract

Twenty monoclonal antibodies were produced against trophozoites of Entamoeba histolytica strains HK‐9 and HM‐1: IMSS. When reactivity to various enteric protozoa was examined by an indirect fluorescence antibody test, 15 of the monoclonal antibodies were strongly reactive with E. histolytica trophozoites. Species‐specific antigens recognized by these monoclonal antibodies were located on the plasma membrane, nucleus, cytoplasm, and cytoskeletal structures of the trophozoites. Two of the remaining five monoclonals reacted strongly with trophozoites of the E. histolytica‐like Laredo strain. The determinant antigen was located in the cytoplasm. The three remaining monoclonal antibodies were found to recognize cross‐reactive antigens between E. histolytica and E. histolytica‐like Laredo, E. hartmanni, E. coli, Dientamoeba fragilis, Giardia lamblia, and Trichomonas hominis. These three antibodies were also reactive with T. vaginalis

Original languageEnglish
Pages (from-to)329-334
Number of pages6
JournalThe Journal of Protozoology
Volume38
Issue number4
DOIs
Publication statusPublished - 1991 Jul

Keywords

  • Indirect fluorescence antibody test
  • Western blotting.

ASJC Scopus subject areas

  • Parasitology

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    TACHIBANA, HIROSHI., KOBAYASHI, SEIKI., NAGAKURA, KOUICHI., KANEDA, YOSHIMASA., & TAKEUCHI, TSUTOMU. (1991). Reactivity of Monoclonal Antibodies to Species‐Specific Antigens of Entamoeba histolytica. The Journal of Protozoology, 38(4), 329-334. https://doi.org/10.1111/j.1550-7408.1991.tb01368.x