Real-time measurement of nitric oxide using a bio-imaging and an electrochemical technique

Yoshiichiro Kitamura, Hiroto Ogawa, Kotaro Oka

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Nitric oxide (NO) is an important mediator responsible for numerous physiological phenomena. Transient levels of NO in biological systems usually range from nanomolar to micromolar concentrations, with a rapid return to basal levels normally seen following these increases. Because NO can diffuse only over a local area in limited time due to such low levels of production and due to its short life-time prior to degradation, high spatial and temporal resolutions are required for direct and continuous NO measurement if the physiological role of NO is to be investigated in any system. For such purposes, analytical methods based on bio-imaging and electrochemical techniques for the measurement of NO are useful. In this paper, we describe the successful application of these methods to a number of biological systems. Specifically, complementary application of these methods demonstrate that it is possible to detect real-time NO production from nervous tissue with high spatial and temporal resolutions.

Original languageEnglish
Pages (from-to)717-724
Number of pages8
JournalTalanta
Volume61
Issue number5
DOIs
Publication statusPublished - 2003 Dec 4

Fingerprint

Electrochemical Techniques
Time measurement
Nitric Oxide
Imaging techniques
Biological systems
Physiological Phenomena
Nerve Tissue
Tissue
Degradation

Keywords

  • Earthworm
  • Endothelial cells
  • NO-specific electrode
  • NO-specific fluorescent dye
  • Ventral nerve cord

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

Real-time measurement of nitric oxide using a bio-imaging and an electrochemical technique. / Kitamura, Yoshiichiro; Ogawa, Hiroto; Oka, Kotaro.

In: Talanta, Vol. 61, No. 5, 04.12.2003, p. 717-724.

Research output: Contribution to journalArticle

Kitamura, Yoshiichiro ; Ogawa, Hiroto ; Oka, Kotaro. / Real-time measurement of nitric oxide using a bio-imaging and an electrochemical technique. In: Talanta. 2003 ; Vol. 61, No. 5. pp. 717-724.
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