Real-time non-invasive in vivo visible light detection of cortical spreading depolarizations in mice

David Y. Chung, Kazutaka Sugimoto, Paul Fischer, Maximilian Böhm, Tsubasa Takizawa, Homa Sadeghian, Andreia Morais, Andrea Harriott, Fumiaki Oka, Tao Qin, Nils Henninger, Mohammad A. Yaseen, Sava Sakadžić, Cenk Ayata

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Cortical spreading depolarization (CSD) is a phenomenon classically associated with migraine aura. CSDs have also been implicated in secondary injury following ischemic stroke, intracerebral hemorrhage, subarachnoid hemorrhage, and traumatic brain injury; however, most investigations involving these disease processes do not account for the occurrence of CSDs. A major barrier to detection of CSDs in experimental models is that currently validated methods are invasive and require specialized equipment and a high level of expertise to implement. New method: We present a low-cost, easy-to-implement approach to the detection of CSDs in the mouse through full-thickness intact skull. Our method uses the optical intrinsic signal from white light illumination (OIS-WL) and allows for real-time in vivo detection of CSDs using readily available USB cameras. Results: OIS-WL detected 100% of CSDs that were seen with simultaneous electrode recording (69 CSDs in 28 mice), laser Doppler flowmetry (82 CSDs in 10 mice), laser speckle flowmetry (68 CSDs in 25 mice), or combined electrode recording plus laser speckle flowmetry (29 CSDs in 20 mice). OIS-WL detected 1 additional CSD that was missed by laser Doppler flowmetry. Comparison with existing methods: : OIS-WL is less invasive than electrophysiological recordings and easier to implement than laser speckle flowmetry. Moreover, it provides excellent spatial and temporal resolution for dynamic imaging of CSDs in the setting of brain injury. Conclusions: Detection of CSDs with an inexpensive USB camera and white light source provides a reliable method for the in vivo and non-invasive detection of CSDs through unaltered mouse skull.

Original languageEnglish
Pages (from-to)143-146
Number of pages4
JournalJournal of Neuroscience Methods
Volume309
DOIs
Publication statusPublished - 2018 Nov 1

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Lighting
Light
Rheology
Laser-Doppler Flowmetry
Lasers
Skull
Electrodes
Migraine with Aura
Cerebral Hemorrhage
Subarachnoid Hemorrhage
Brain Injuries
Theoretical Models
Stroke
Costs and Cost Analysis
Equipment and Supplies
Wounds and Injuries

Keywords

  • Cortical spreading depression
  • Migraine
  • Non-invasive
  • Optical intrinsic signal imaging
  • Stroke

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Real-time non-invasive in vivo visible light detection of cortical spreading depolarizations in mice. / Chung, David Y.; Sugimoto, Kazutaka; Fischer, Paul; Böhm, Maximilian; Takizawa, Tsubasa; Sadeghian, Homa; Morais, Andreia; Harriott, Andrea; Oka, Fumiaki; Qin, Tao; Henninger, Nils; Yaseen, Mohammad A.; Sakadžić, Sava; Ayata, Cenk.

In: Journal of Neuroscience Methods, Vol. 309, 01.11.2018, p. 143-146.

Research output: Contribution to journalArticle

Chung, DY, Sugimoto, K, Fischer, P, Böhm, M, Takizawa, T, Sadeghian, H, Morais, A, Harriott, A, Oka, F, Qin, T, Henninger, N, Yaseen, MA, Sakadžić, S & Ayata, C 2018, 'Real-time non-invasive in vivo visible light detection of cortical spreading depolarizations in mice', Journal of Neuroscience Methods, vol. 309, pp. 143-146. https://doi.org/10.1016/j.jneumeth.2018.09.001
Chung, David Y. ; Sugimoto, Kazutaka ; Fischer, Paul ; Böhm, Maximilian ; Takizawa, Tsubasa ; Sadeghian, Homa ; Morais, Andreia ; Harriott, Andrea ; Oka, Fumiaki ; Qin, Tao ; Henninger, Nils ; Yaseen, Mohammad A. ; Sakadžić, Sava ; Ayata, Cenk. / Real-time non-invasive in vivo visible light detection of cortical spreading depolarizations in mice. In: Journal of Neuroscience Methods. 2018 ; Vol. 309. pp. 143-146.
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abstract = "Background: Cortical spreading depolarization (CSD) is a phenomenon classically associated with migraine aura. CSDs have also been implicated in secondary injury following ischemic stroke, intracerebral hemorrhage, subarachnoid hemorrhage, and traumatic brain injury; however, most investigations involving these disease processes do not account for the occurrence of CSDs. A major barrier to detection of CSDs in experimental models is that currently validated methods are invasive and require specialized equipment and a high level of expertise to implement. New method: We present a low-cost, easy-to-implement approach to the detection of CSDs in the mouse through full-thickness intact skull. Our method uses the optical intrinsic signal from white light illumination (OIS-WL) and allows for real-time in vivo detection of CSDs using readily available USB cameras. Results: OIS-WL detected 100{\%} of CSDs that were seen with simultaneous electrode recording (69 CSDs in 28 mice), laser Doppler flowmetry (82 CSDs in 10 mice), laser speckle flowmetry (68 CSDs in 25 mice), or combined electrode recording plus laser speckle flowmetry (29 CSDs in 20 mice). OIS-WL detected 1 additional CSD that was missed by laser Doppler flowmetry. Comparison with existing methods: : OIS-WL is less invasive than electrophysiological recordings and easier to implement than laser speckle flowmetry. Moreover, it provides excellent spatial and temporal resolution for dynamic imaging of CSDs in the setting of brain injury. Conclusions: Detection of CSDs with an inexpensive USB camera and white light source provides a reliable method for the in vivo and non-invasive detection of CSDs through unaltered mouse skull.",
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T1 - Real-time non-invasive in vivo visible light detection of cortical spreading depolarizations in mice

AU - Chung, David Y.

AU - Sugimoto, Kazutaka

AU - Fischer, Paul

AU - Böhm, Maximilian

AU - Takizawa, Tsubasa

AU - Sadeghian, Homa

AU - Morais, Andreia

AU - Harriott, Andrea

AU - Oka, Fumiaki

AU - Qin, Tao

AU - Henninger, Nils

AU - Yaseen, Mohammad A.

AU - Sakadžić, Sava

AU - Ayata, Cenk

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N2 - Background: Cortical spreading depolarization (CSD) is a phenomenon classically associated with migraine aura. CSDs have also been implicated in secondary injury following ischemic stroke, intracerebral hemorrhage, subarachnoid hemorrhage, and traumatic brain injury; however, most investigations involving these disease processes do not account for the occurrence of CSDs. A major barrier to detection of CSDs in experimental models is that currently validated methods are invasive and require specialized equipment and a high level of expertise to implement. New method: We present a low-cost, easy-to-implement approach to the detection of CSDs in the mouse through full-thickness intact skull. Our method uses the optical intrinsic signal from white light illumination (OIS-WL) and allows for real-time in vivo detection of CSDs using readily available USB cameras. Results: OIS-WL detected 100% of CSDs that were seen with simultaneous electrode recording (69 CSDs in 28 mice), laser Doppler flowmetry (82 CSDs in 10 mice), laser speckle flowmetry (68 CSDs in 25 mice), or combined electrode recording plus laser speckle flowmetry (29 CSDs in 20 mice). OIS-WL detected 1 additional CSD that was missed by laser Doppler flowmetry. Comparison with existing methods: : OIS-WL is less invasive than electrophysiological recordings and easier to implement than laser speckle flowmetry. Moreover, it provides excellent spatial and temporal resolution for dynamic imaging of CSDs in the setting of brain injury. Conclusions: Detection of CSDs with an inexpensive USB camera and white light source provides a reliable method for the in vivo and non-invasive detection of CSDs through unaltered mouse skull.

AB - Background: Cortical spreading depolarization (CSD) is a phenomenon classically associated with migraine aura. CSDs have also been implicated in secondary injury following ischemic stroke, intracerebral hemorrhage, subarachnoid hemorrhage, and traumatic brain injury; however, most investigations involving these disease processes do not account for the occurrence of CSDs. A major barrier to detection of CSDs in experimental models is that currently validated methods are invasive and require specialized equipment and a high level of expertise to implement. New method: We present a low-cost, easy-to-implement approach to the detection of CSDs in the mouse through full-thickness intact skull. Our method uses the optical intrinsic signal from white light illumination (OIS-WL) and allows for real-time in vivo detection of CSDs using readily available USB cameras. Results: OIS-WL detected 100% of CSDs that were seen with simultaneous electrode recording (69 CSDs in 28 mice), laser Doppler flowmetry (82 CSDs in 10 mice), laser speckle flowmetry (68 CSDs in 25 mice), or combined electrode recording plus laser speckle flowmetry (29 CSDs in 20 mice). OIS-WL detected 1 additional CSD that was missed by laser Doppler flowmetry. Comparison with existing methods: : OIS-WL is less invasive than electrophysiological recordings and easier to implement than laser speckle flowmetry. Moreover, it provides excellent spatial and temporal resolution for dynamic imaging of CSDs in the setting of brain injury. Conclusions: Detection of CSDs with an inexpensive USB camera and white light source provides a reliable method for the in vivo and non-invasive detection of CSDs through unaltered mouse skull.

KW - Cortical spreading depression

KW - Migraine

KW - Non-invasive

KW - Optical intrinsic signal imaging

KW - Stroke

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