Real-time visualization of oxyradical burst from single neutrophil by using ultrasensitive video intensifier microscopy

Makoto Suematsu, Chikara Oshio, Soichiro Miura, Masaharu Tsuchiya

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Dynamics of oxyradical burst from single human neutrophils stimulated by opsonized zymosan was visualized by using an ultrasensitive video intensifier microscopy in the presence of chemiluminescence probe. Luminol-dependent photonic burst activities were clearly corresponding to the distribution of zymosan-treated neutrophils. Heterogeneity of photon-bursting period and the maximum photonic intensity among reactive cells was demonstrated. This may reflect functional heterogeneity of the ability to release oxyradicals among neutrophils.

Original languageEnglish
Pages (from-to)1106-1110
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume149
Issue number3
DOIs
Publication statusPublished - 1987 Dec 31

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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