Real-time visualization of oxyradical burst from single neutrophil by using ultrasensitive video intensifier microscopy

Makoto Suematsu; Chikara Oshio; Soichiro Miura; Masaharu Tsuchiya

doi:10.1016/0006-291X(87)90522-5

Real-time visualization of oxyradical burst from single neutrophil by using ultrasensitive video intensifier microscopy

Makoto Suematsu, Chikara Oshio, Soichiro Miura, Masaharu Tsuchiya

Department of Biochemistry

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Dynamics of oxyradical burst from single human neutrophils stimulated by opsonized zymosan was visualized by using an ultrasensitive video intensifier microscopy in the presence of chemiluminescence probe. Luminol-dependent photonic burst activities were clearly corresponding to the distribution of zymosan-treated neutrophils. Heterogeneity of photon-bursting period and the maximum photonic intensity among reactive cells was demonstrated. This may reflect functional heterogeneity of the ability to release oxyradicals among neutrophils.

Original language	English
Pages (from-to)	1106-1110
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	149
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(87)90522-5
Publication status	Published - 1987 Dec 31

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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AU - Miura, Soichiro

AU - Tsuchiya, Masaharu

PY - 1987/12/31

Y1 - 1987/12/31

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AB - Dynamics of oxyradical burst from single human neutrophils stimulated by opsonized zymosan was visualized by using an ultrasensitive video intensifier microscopy in the presence of chemiluminescence probe. Luminol-dependent photonic burst activities were clearly corresponding to the distribution of zymosan-treated neutrophils. Heterogeneity of photon-bursting period and the maximum photonic intensity among reactive cells was demonstrated. This may reflect functional heterogeneity of the ability to release oxyradicals among neutrophils.

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Real-time visualization of oxyradical burst from single neutrophil by using ultrasensitive video intensifier microscopy

Abstract

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