Real-time visualization of oxyradical burst from single neutrophil by using ultrasensitive video intensifier microscopy

Makoto Suematsu, Chikara Oshio, Soichiro Miura, Masaharu Tsuchiya

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Dynamics of oxyradical burst from single human neutrophils stimulated by opsonized zymosan was visualized by using an ultrasensitive video intensifier microscopy in the presence of chemiluminescence probe. Luminol-dependent photonic burst activities were clearly corresponding to the distribution of zymosan-treated neutrophils. Heterogeneity of photon-bursting period and the maximum photonic intensity among reactive cells was demonstrated. This may reflect functional heterogeneity of the ability to release oxyradicals among neutrophils.

Original languageEnglish
Pages (from-to)1106-1110
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number3
Publication statusPublished - 1987 Dec 31


ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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