TY - JOUR
T1 - Recent advances in detection, isolation, and imaging techniques for sulfane sulfur-containing biomolecules
AU - Echizen, Honami
AU - Sasaki, Eita
AU - Hanaoka, Kenjiro
N1 - Funding Information:
Funding: This work was supported in part by grants from JSPS KAKENHI, Grant Numbers 16H05099, 18H04609, 20H04767, and 20H02701 to K.H., and SENTAN, JST to K.H., who was also supported by Hoansha Foundation and Daiichi Sankyo Foundation of Life Science.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/11
Y1 - 2021/11
N2 - Hydrogen sulfide and its oxidation products are involved in many biological processes, and sulfane sulfur compounds, which contain sulfur atoms bonded to other sulfur atom(s), as found in hydropersulfides (R-S-SH), polysulfides (R-S-Sn-S-R), hydrogen polysulfides (H2Sn), etc., have at-tracted increasing interest. To characterize their physiological and pathophysiological roles, selective detection techniques are required. Classically, sulfane sulfur compounds can be detected by cyanolysis, involving nucleophilic attack by cyanide ion to cleave the sulfur–sulfur bonds. The generated thiocyanate reacts with ferric ion, and the resulting ferric thiocyanate complex can be easily detected by absorption spectroscopy. Recent exploration of the properties of sulfane sulfur compounds as both nucleophiles and electrophiles has led to the development of various chemical techniques for detection, isolation, and bioimaging of sulfane sulfur compounds in biological samples. These include tag-switch techniques, LC-MS/MS, Raman spectroscopy, and fluorescent probes. Herein, we present an overview of the techniques available for specific detection of sulfane sulfur species in biological contexts.
AB - Hydrogen sulfide and its oxidation products are involved in many biological processes, and sulfane sulfur compounds, which contain sulfur atoms bonded to other sulfur atom(s), as found in hydropersulfides (R-S-SH), polysulfides (R-S-Sn-S-R), hydrogen polysulfides (H2Sn), etc., have at-tracted increasing interest. To characterize their physiological and pathophysiological roles, selective detection techniques are required. Classically, sulfane sulfur compounds can be detected by cyanolysis, involving nucleophilic attack by cyanide ion to cleave the sulfur–sulfur bonds. The generated thiocyanate reacts with ferric ion, and the resulting ferric thiocyanate complex can be easily detected by absorption spectroscopy. Recent exploration of the properties of sulfane sulfur compounds as both nucleophiles and electrophiles has led to the development of various chemical techniques for detection, isolation, and bioimaging of sulfane sulfur compounds in biological samples. These include tag-switch techniques, LC-MS/MS, Raman spectroscopy, and fluorescent probes. Herein, we present an overview of the techniques available for specific detection of sulfane sulfur species in biological contexts.
KW - Fluorescent probes
KW - Hydrogen persulfide
KW - Hydrogen sulfide
KW - Polysulfide
KW - Sulfane sulfur
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U2 - 10.3390/biom11111553
DO - 10.3390/biom11111553
M3 - Review article
C2 - 34827552
AN - SCOPUS:85117284556
VL - 11
JO - Biomolecules
JF - Biomolecules
SN - 2218-273X
IS - 11
M1 - 1553
ER -