TY - JOUR
T1 - Recent advances in probe design to detect reactive sulfur species and in the chemical reactions employed for fluorescence switching
AU - Echizen, Honami
AU - Hanaoka, Kenjiro
N1 - Funding Information:
This work was supported in part by grants by JSPS KAKENHI Grant Numbers 16H05099, 18H04609, and 20H04767 to KH, the Japan Agency for Medical Research and Development (AMED) to KH (JP18fm0208029), and SENTAN, JST to KH, who was also supported by Hoansha Foundation, Daiichi Sankyo Foundation of Life Science, a grant JSPS Core-to-Core program, A. Advanced Research Networks, and a Grant-in-Aid for Scientific Research on Innovative Areas “Singularity Biology (No. 8007)” (JP19H05414 to KH) of The Ministry of Education, Culture, Sports, Science, and Technology, Japan.
Publisher Copyright:
©2021 JCBN
PY - 2021/1
Y1 - 2021/1
N2 - Reactive sulfur species, including hydrogen sulfide, hydropersulfide, and polysulfide, have many roles in biological systems. For example, hydrogen sulfide is involved in the relaxation of vascular smooth muscles and mediation of neurotransmission, while sulfane sulfur, which exists in cysteine persulfide/polysulfide, and glutathione persulfide/polysulfide, is involved in physiological antioxidation and cytoprotection mechanisms. Fluorescence imaging is well suited for real-time monitoring of reactive sulfur species in living cells, and many fluorescent probes for reactive sulfur species have been reported. In such probes, the choice of detection chemistry is extremely important, not only to achieve effective fluorescence switching and high selectivity, but also because the reactions may be applicable to develop other chemical tools, such as reactive sulfur species donors/scavengers. Here, we present an overview of both widely used and recently developed fluorescent probes for reactive sulfur species, focusing especially on the chemical reactions employed in them for fluorescence switching. We also briefly introduce some applications of fluorescent probes for hydrogen sulfide and sulfane sulfur.
AB - Reactive sulfur species, including hydrogen sulfide, hydropersulfide, and polysulfide, have many roles in biological systems. For example, hydrogen sulfide is involved in the relaxation of vascular smooth muscles and mediation of neurotransmission, while sulfane sulfur, which exists in cysteine persulfide/polysulfide, and glutathione persulfide/polysulfide, is involved in physiological antioxidation and cytoprotection mechanisms. Fluorescence imaging is well suited for real-time monitoring of reactive sulfur species in living cells, and many fluorescent probes for reactive sulfur species have been reported. In such probes, the choice of detection chemistry is extremely important, not only to achieve effective fluorescence switching and high selectivity, but also because the reactions may be applicable to develop other chemical tools, such as reactive sulfur species donors/scavengers. Here, we present an overview of both widely used and recently developed fluorescent probes for reactive sulfur species, focusing especially on the chemical reactions employed in them for fluorescence switching. We also briefly introduce some applications of fluorescent probes for hydrogen sulfide and sulfane sulfur.
KW - Fluorescent probe
KW - HSNO
KW - Hydrogen sulfide
KW - Reactive sulfur species
KW - Sulfane sulfur
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U2 - 10.3164/JCBN.20-18
DO - 10.3164/JCBN.20-18
M3 - Article
AN - SCOPUS:85100079021
VL - 68
SP - 9
EP - 17
JO - Journal of Clinical Biochemistry and Nutrition
JF - Journal of Clinical Biochemistry and Nutrition
SN - 0912-0009
IS - 1
ER -