Reck negatively regulates matrix metalloproteinase-9 transcription

Satoshi Takagi, Siro Simizu, Hiroyuki Osada

Research output: Contribution to journalArticlepeer-review

74 Citations (Scopus)

Abstract

RECK, a glycosylphosphatidylinositol-anchored glycoprotein, inhibits the enzymatic activities of some matrix metallopro-teinases (MMP), thereby suppressing tumor cell metastasis; however, the detailed mechanism is still obscure. In this study, we compared the gene expression profiles between mock- and RECK-transfected HT1080 cells and showed that RECK decreases MMP-9 mRNA levels but not other MMP mRNA levels. Moreover, treatment with RECK-specific siRNA increased MMP-9 mRNA in RECK-expressing cells. The promoter assay showed that MMP-9 promoter activity was suppressed by RECK and that RECK-mediated suppression of MMP-9 promoter activity requires 12-0-tetradecanoylphorbol-13- acetate-responsive element (TRE) and κB sites. Moreover, the binding ability of Fra-1 and c-Jun to TRE within the MMP-9 promoter region was suppressed by RECK. Thus, these results show that RECK is a negative regulator of MMP-9 transcription.

Original languageEnglish
Pages (from-to)1502-1508
Number of pages7
JournalCancer Research
Volume69
Issue number4
DOIs
Publication statusPublished - 2009 Feb 15
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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