Recognition of consensus CHO structure in ligands for selectins by novel antibody against sialyl Lewis X

T. Tamatani, M. Suematsu, K. Tezuka, N. Hanzawa, T. Tsuji, Y. Ishimura, R. Kannagi, S. Toyoshima, M. Homma

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

The selectins (L, E, and P) play an important role in the earliest events of the inflammatory response, leading to the 'rolling' phenomenon. All selectins react with sialyl Lewis X (SLe(x)) in vitro, possibly suggesting that their ligands have a consensus structure. 2H5 is a monoclonal antibody against SLe(x) that blocks L-selectin-mediated adhesion. 2H5 inhibited adhesion of HL-60 cells to P- and E-selectin-producing COS cells in vitro and immunoprecipitated a P-selectin glycoprotein ligand-1-like glycoprotein from HL-60 cell lysate, suggesting that it recognizes a functional consensus structure on the ligands for all selectins. 2H5 reacted not only with human but also with rat and mouse neutrophils. 2H5 is the first antibody against SLe(x) that recognizes neutrophils of nonhuman mammals. The carbohydrate structure recognized by 2H5 was present nut only on high endothelial venules of rat lymphoid organs but also on the endothelial cells of nonlymphoid organs. Furthermore, administration of the antibody markedly inhibited L- and P-selectin-mediated neutrophil rolling and adhesion in rat mesenteric venules in vivo. These results provide evidence for the presence of a consensus carbohydrate structure on the ligands fur all selectins. The consensus structure thus has the potential to serve as a therapeutic target.

Original languageEnglish
Pages (from-to)H1282-H1287
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume269
Issue number4 38-4
DOIs
Publication statusPublished - 1995 Jan 1
Externally publishedYes

Keywords

  • cell adhesion
  • cell rolling
  • monoclonal antibody
  • oligosaccharide

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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