Recognition of shared melanoma antigens by human tumor-infiltrating lymphocytes

Suzanne L. Topalian, Sophia S. Horn, Yutaka Kawakami, Marie Mancini, Douglas J. Schwartzentruber, Rina Zakut, Steven A. Rosenberg

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We have established that melanomas express shared tumor antigens (Ags) that can be recognized by T cells if presented in the context of self-MHC molecules. Tumor-infiltrating lymphocytes (TILs) from six melanoma patients were tested for lysis of large panels of HLA-matched or unmatched targets representing a variety of tissue types. Lysis was specific for allogeneic melanomas sharing at least one HLA-A, -B, or -C Ag with TILs, and demonstrated commonly expressed tumor Ags. Similar findings were obtained when cytokine secretion by TILs was used to indicate specific Ag recognition. Transfection of the HLA-A2.1 gene into HLA-A2- melanoma lines conferred susceptibility to lysis by HLA-A2 restricted melanoma TILs, demonstrating expression of common tumor Ags among patients of diverse HLA types. These findings have important implications for developing broadly applicable cancer immunotherapies such as vaccines.

Original languageEnglish
Pages (from-to)203-206
Number of pages4
JournalJournal of Immunotherapy
Volume12
Issue number3
DOIs
Publication statusPublished - 1992 Oct

    Fingerprint

Keywords

  • Melanoma
  • Tumor-infiltrating lymphocytes
  • Tumor-specific antigens

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology
  • Cancer Research

Cite this

Topalian, S. L., Horn, S. S., Kawakami, Y., Mancini, M., Schwartzentruber, D. J., Zakut, R., & Rosenberg, S. A. (1992). Recognition of shared melanoma antigens by human tumor-infiltrating lymphocytes. Journal of Immunotherapy, 12(3), 203-206. https://doi.org/10.1097/00002371-199210000-00013