Reconstitution activity of hypoxic cultured human cord blood CD34-positive cells in NOG mice

Haruko Shima, Keiyo Takubo, Hiroko Iwasaki, Hiroki Yoshihara, Yumiko Gomei, Kentaro Hosokawa, Fumio Arai, Takao Takahashi, Toshio Suda

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Hematopoietic stem cells (HSCs) reside in hypoxic areas of the bone marrow. However, the role of hypoxia in the maintenance of HSCs has not been fully characterized. We performed xenotransplantation of human cord blood cells cultured in hypoxic or normoxic conditions into adult NOD/SCID/IL-2Rγnull (NOG) mice. Hypoxic culture (1% O2) for 6 days efficiently supported the maintenance of HSCs, although cell proliferation was suppressed compared to the normoxic culture. In contrast, hypoxia did not affect in vitro colony-forming ability. Upregulation of a cell cycle inhibitor, p21, was observed in hypoxic culture. Immunohistochemical analysis of recipient bone marrow revealed that engrafted CD34+CD38- cord blood HSCs were hypoxic. Taken together, these results demonstrate the significance of hypoxia in the maintenance of quiescent human cord blood HSCs.

Original languageEnglish
Pages (from-to)467-472
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume378
Issue number3
DOIs
Publication statusPublished - 2009 Jan 16

Keywords

  • Hematopoietic stem cell
  • Human cord blood cells
  • Hypoxia
  • NOD/SCID/IL-2Rγ mouse
  • Niche
  • Quiescence
  • Reactive oxygen species
  • p21

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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