TY - JOUR
T1 - Reconstitution activity of hypoxic cultured human cord blood CD34-positive cells in NOG mice
AU - Shima, Haruko
AU - Takubo, Keiyo
AU - Iwasaki, Hiroko
AU - Yoshihara, Hiroki
AU - Gomei, Yumiko
AU - Hosokawa, Kentaro
AU - Arai, Fumio
AU - Takahashi, Takao
AU - Suda, Toshio
PY - 2009/1/16
Y1 - 2009/1/16
N2 - Hematopoietic stem cells (HSCs) reside in hypoxic areas of the bone marrow. However, the role of hypoxia in the maintenance of HSCs has not been fully characterized. We performed xenotransplantation of human cord blood cells cultured in hypoxic or normoxic conditions into adult NOD/SCID/IL-2Rγnull (NOG) mice. Hypoxic culture (1% O2) for 6 days efficiently supported the maintenance of HSCs, although cell proliferation was suppressed compared to the normoxic culture. In contrast, hypoxia did not affect in vitro colony-forming ability. Upregulation of a cell cycle inhibitor, p21, was observed in hypoxic culture. Immunohistochemical analysis of recipient bone marrow revealed that engrafted CD34+CD38- cord blood HSCs were hypoxic. Taken together, these results demonstrate the significance of hypoxia in the maintenance of quiescent human cord blood HSCs.
AB - Hematopoietic stem cells (HSCs) reside in hypoxic areas of the bone marrow. However, the role of hypoxia in the maintenance of HSCs has not been fully characterized. We performed xenotransplantation of human cord blood cells cultured in hypoxic or normoxic conditions into adult NOD/SCID/IL-2Rγnull (NOG) mice. Hypoxic culture (1% O2) for 6 days efficiently supported the maintenance of HSCs, although cell proliferation was suppressed compared to the normoxic culture. In contrast, hypoxia did not affect in vitro colony-forming ability. Upregulation of a cell cycle inhibitor, p21, was observed in hypoxic culture. Immunohistochemical analysis of recipient bone marrow revealed that engrafted CD34+CD38- cord blood HSCs were hypoxic. Taken together, these results demonstrate the significance of hypoxia in the maintenance of quiescent human cord blood HSCs.
KW - Hematopoietic stem cell
KW - Human cord blood cells
KW - Hypoxia
KW - NOD/SCID/IL-2Rγ mouse
KW - Niche
KW - Quiescence
KW - Reactive oxygen species
KW - p21
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U2 - 10.1016/j.bbrc.2008.11.056
DO - 10.1016/j.bbrc.2008.11.056
M3 - Article
C2 - 19032938
AN - SCOPUS:57749203149
SN - 0006-291X
VL - 378
SP - 467
EP - 472
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -