Reconstitution of immune systems in RAG2(-/-) mice by transfer with interleukin-12-induced splenic hematopoietic progenitor cells

Takashi Yahata, Kiyoshi Ando, Kazuhito Watanabe, Takehiko Mori, Akio Ohta, Yasushi Ohmi, Kenji Iwakabe, Soichi Kuge, Minoru Nakui, Mamoru Ito, Sonoko Habu, Shuhei Yasuda, Takashi Nishimura

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The administration of a high dose of IL-12 into the mice resulted in the induction of splenomegaly. From the flow cytometry analysis of cellularity in an enlarged spleen, it was demonstrated that Thy1.2-CD45RB-c-Kit+Sca- 1+Lin- hematopoietic progenitor cells markedly increased in IL-12- administered mouse spleen compared with untreated mouse spleen. The IL-12- induced hematopoietic progenitor cells showed a greatly enhanced colony- forming activity in CFU-granulocyte/macrophage (CFU-GM), blast-forming units- erythroid (BFU-E) and CFU-spleen (CFU-S) assay. Moreover, it was initially demonstrated that the transfer of IL-12-induced splenic hematopoietic progenitor cells into immunodeficient RAG2(-/-) mice caused a complete reconstitution of their immune functions including T- and B-cell-mediated immunity. Thus, the evidence that IL-12 has a capability of inducing hematopoietic progenitor cells possessing stem cell-like activity in vivo, indicated another important immunomodulating activity of IL-12 in immunotherapy.

Original languageEnglish
Pages (from-to)165-170
Number of pages6
JournalImmunology Letters
Volume62
Issue number3
DOIs
Publication statusPublished - 1998 Jul 1

Keywords

  • Colony-forming activity
  • Hematopoietic progenitor, RAG2(-/-) mice
  • IL-12

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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