Reconstruction of 3D stacked hepatocyte tissues using degradable, microporous poly(d,l-lactide-co-glycolide) membranes

Junichi Kasuya, Ryo Sudo, Ryu Tamogami, Genta Masuda, Toshihiro Mitaka, Mariko Ikeda, Kazuo Tanishita

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

There is great demand for constructing well-organized three-dimensional (3D) tissues invitro. Here, we developed a 3D stacked culture method using biodegradable poly(d,l-lactide-co- glycolide) (PLGA) membranes with defined topography. Pore size and porosity of the membranes can be controlled by changing the moisture content during fabrication. The optimized membrane served as a scaffold to manipulate small hepatocyte (SH) layers when they were stacked, while it degraded after stacking, resulting in the reorganization of the cells into a 3D stacked structure. Immunofluorescent staining for domain markers of cell polarity and electron microscopy confirmed that the cells in the 3D stacked structures recovered polarity. Furthermore, the cells exhibited improved liver-specific function as compared with cells in a monolayer. This 3D stacked culture may enable reconstruction of multilayered hepatic tissues with highly differentiated functions invitro.

Original languageEnglish
Pages (from-to)2693-2700
Number of pages8
JournalBiomaterials
Volume33
Issue number9
DOIs
Publication statusPublished - 2012 Mar

Fingerprint

Hepatocytes
Tissue
Membranes
Scaffolds
Liver
Cell Polarity
Topography
Electron microscopy
Pore size
Porosity
Monolayers
Moisture
Fabrication
Electron Microscopy
Staining and Labeling
dilactide
polylactic acid-polyglycolic acid copolymer

Keywords

  • Biodegradation
  • Cell culture
  • Hepatocyte
  • Liver
  • Polyglycolic acid
  • Polylactic acid

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Reconstruction of 3D stacked hepatocyte tissues using degradable, microporous poly(d,l-lactide-co-glycolide) membranes. / Kasuya, Junichi; Sudo, Ryo; Tamogami, Ryu; Masuda, Genta; Mitaka, Toshihiro; Ikeda, Mariko; Tanishita, Kazuo.

In: Biomaterials, Vol. 33, No. 9, 03.2012, p. 2693-2700.

Research output: Contribution to journalArticle

Kasuya, Junichi ; Sudo, Ryo ; Tamogami, Ryu ; Masuda, Genta ; Mitaka, Toshihiro ; Ikeda, Mariko ; Tanishita, Kazuo. / Reconstruction of 3D stacked hepatocyte tissues using degradable, microporous poly(d,l-lactide-co-glycolide) membranes. In: Biomaterials. 2012 ; Vol. 33, No. 9. pp. 2693-2700.
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