TY - JOUR
T1 - Redifferentiation of dedifferentiated chondrocytes and chondrogenesis of human bone marrow stromal cells via chondrosphere formation with expression profiling by large-scale cDNA analysis
AU - Imabayashi, Hideaki
AU - Mori, Taisuke
AU - Gojo, Satoshi
AU - Kiyono, Tohru
AU - Sugiyama, Tomoyasu
AU - Irie, Ryotaro
AU - Isogai, Takao
AU - Hata, Jun Ichi
AU - Toyama, Yoshiaki
AU - Umezawa, Akihiro
PY - 2003/8/1
Y1 - 2003/8/1
N2 - Characterization of dedifferentiated chondrocytes (DECs) and mesenchymal stem cells capable of differentiating into chondrocytes is of biological and clinical interest. We isolated DECs and bone marrow stromal cells (BMSCs), H4-1 and H3-4, and demonstrated that the cells started to produce extracellular matrices, such as type II collagen and aggrecan, at an early stage of chondrosphere formation. Furthermore, cDNA sequencing of cDNA libraries constricted by the oligocapping method was performed to analyze difference in mRNA expression profiling between DECs and marrow stromal cells. Upon redifferentiation of DECs, cartilage-related extracellular matrix genes, such as those encoding leucine-rich small proteoglycans, cartilage oligomeric matrix protein, and chitinase 3-like 1 (cartilage glycoprotein-39), were highly expressed. Growth factors such as FGF7 and CTGF were detected at a high frequency in the growth stage of monolayer stromal cultures. By combining the expression profile and flow cytometry, we demonstrated that isolated stromal cells, defined by CD34-, c-kit-, and CD140α- or low, have chondrogenic potential. The newly established human mesenchymal cells with expression profiling provide a powerful model for a study of chondrogenic differentiation and further understanding of cartilage regeneration in the means of redifferentiated DECs and BMSCs.
AB - Characterization of dedifferentiated chondrocytes (DECs) and mesenchymal stem cells capable of differentiating into chondrocytes is of biological and clinical interest. We isolated DECs and bone marrow stromal cells (BMSCs), H4-1 and H3-4, and demonstrated that the cells started to produce extracellular matrices, such as type II collagen and aggrecan, at an early stage of chondrosphere formation. Furthermore, cDNA sequencing of cDNA libraries constricted by the oligocapping method was performed to analyze difference in mRNA expression profiling between DECs and marrow stromal cells. Upon redifferentiation of DECs, cartilage-related extracellular matrix genes, such as those encoding leucine-rich small proteoglycans, cartilage oligomeric matrix protein, and chitinase 3-like 1 (cartilage glycoprotein-39), were highly expressed. Growth factors such as FGF7 and CTGF were detected at a high frequency in the growth stage of monolayer stromal cultures. By combining the expression profile and flow cytometry, we demonstrated that isolated stromal cells, defined by CD34-, c-kit-, and CD140α- or low, have chondrogenic potential. The newly established human mesenchymal cells with expression profiling provide a powerful model for a study of chondrogenic differentiation and further understanding of cartilage regeneration in the means of redifferentiated DECs and BMSCs.
KW - Chondrocytes
KW - Dedifferentiation
KW - Marrow stromal cells
KW - Redifferentiation
KW - Regeneration
KW - Stem cells
KW - Transplantation
UR - http://www.scopus.com/inward/record.url?scp=0038149011&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0038149011&partnerID=8YFLogxK
U2 - 10.1016/S0014-4827(03)00130-7
DO - 10.1016/S0014-4827(03)00130-7
M3 - Article
C2 - 12878157
AN - SCOPUS:0038149011
VL - 288
SP - 35
EP - 50
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 1
ER -