Redifferentiation of dedifferentiated chondrocytes and chondrogenesis of human bone marrow stromal cells via chondrosphere formation with expression profiling by large-scale cDNA analysis

Hideaki Imabayashi, Taisuke Mori, Satoshi Gojo, Tohru Kiyono, Tomoyasu Sugiyama, Ryotaro Irie, Takao Isogai, Jun Ichi Hata, Yoshiaki Toyama, Akihiro Umezawa

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Characterization of dedifferentiated chondrocytes (DECs) and mesenchymal stem cells capable of differentiating into chondrocytes is of biological and clinical interest. We isolated DECs and bone marrow stromal cells (BMSCs), H4-1 and H3-4, and demonstrated that the cells started to produce extracellular matrices, such as type II collagen and aggrecan, at an early stage of chondrosphere formation. Furthermore, cDNA sequencing of cDNA libraries constricted by the oligocapping method was performed to analyze difference in mRNA expression profiling between DECs and marrow stromal cells. Upon redifferentiation of DECs, cartilage-related extracellular matrix genes, such as those encoding leucine-rich small proteoglycans, cartilage oligomeric matrix protein, and chitinase 3-like 1 (cartilage glycoprotein-39), were highly expressed. Growth factors such as FGF7 and CTGF were detected at a high frequency in the growth stage of monolayer stromal cultures. By combining the expression profile and flow cytometry, we demonstrated that isolated stromal cells, defined by CD34-, c-kit-, and CD140α- or low, have chondrogenic potential. The newly established human mesenchymal cells with expression profiling provide a powerful model for a study of chondrogenic differentiation and further understanding of cartilage regeneration in the means of redifferentiated DECs and BMSCs.

Original languageEnglish
Pages (from-to)35-50
Number of pages16
JournalExperimental Cell Research
Volume288
Issue number1
DOIs
Publication statusPublished - 2003 Aug 1

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Chondrogenesis
Chondrocytes
Mesenchymal Stromal Cells
Complementary DNA
Stromal Cells
Cartilage
Extracellular Matrix
Cartilage Oligomeric Matrix Protein
Aggrecans
Chitinases
Collagen Type II
Gene Library
Regeneration
Intercellular Signaling Peptides and Proteins
Flow Cytometry
Bone Marrow
Messenger RNA
Growth
Genes

Keywords

  • Chondrocytes
  • Dedifferentiation
  • Marrow stromal cells
  • Redifferentiation
  • Regeneration
  • Stem cells
  • Transplantation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Redifferentiation of dedifferentiated chondrocytes and chondrogenesis of human bone marrow stromal cells via chondrosphere formation with expression profiling by large-scale cDNA analysis. / Imabayashi, Hideaki; Mori, Taisuke; Gojo, Satoshi; Kiyono, Tohru; Sugiyama, Tomoyasu; Irie, Ryotaro; Isogai, Takao; Hata, Jun Ichi; Toyama, Yoshiaki; Umezawa, Akihiro.

In: Experimental Cell Research, Vol. 288, No. 1, 01.08.2003, p. 35-50.

Research output: Contribution to journalArticle

Imabayashi, Hideaki ; Mori, Taisuke ; Gojo, Satoshi ; Kiyono, Tohru ; Sugiyama, Tomoyasu ; Irie, Ryotaro ; Isogai, Takao ; Hata, Jun Ichi ; Toyama, Yoshiaki ; Umezawa, Akihiro. / Redifferentiation of dedifferentiated chondrocytes and chondrogenesis of human bone marrow stromal cells via chondrosphere formation with expression profiling by large-scale cDNA analysis. In: Experimental Cell Research. 2003 ; Vol. 288, No. 1. pp. 35-50.
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