Reduced p21(WAF1/CIP1) expression and p53 mutation in hepatocellular carcinomas

A. I Min Hui, Yae Kanai, Michiie Sakamoto, Hitoshi Tsuda, Setsuo Hirohashi

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Abstract

p21(WAF1/CIP1) is a universal cyclin-dependent kinase (cdk) inhibitor, the expression of which is regulated by p53-dependent and p53-independent pathways. We examined p21(WAF1/CIP1) expression in and p53 status of 21 primary hepatocellular carcinomas (HCCs) by reverse-transcriptase polymerase chain reaction (RT-PCR) and by PCR single-strand conformation polymorphism (PCR-SSCP) analysis. p21(WAF1/CIP1) messenger RNA expression was reduced markedly in 8 of 21 HCCs (38.1%) and 5 of these 8 HCCs bore p53 mutations. The relative p21(WAF1/CIP1) messenger RNA expression value of HCCs with p53 mutations (.73 ± .13 U, n = 6) was significantly lower than that of HCCs with wild-type p53 (1.00 ± 0.21 U, n = 14; P < .01). The p21(WAF1/CIP1) expression levels in cancerous tissues (.73 ± .13 U) were significantly reduced in comparison with those in noncancerous tissues (.97 ± .13 U) (P < .01) in the 6 cases with p53 mutations. These data indicate that p21(WAF1/CIP1) expression in HCCs is predominantly regulated by dependence on p53 and that reduced p21(WAF1/CIP1) expression may participate in hepatocarcinogenesis.

Original languageEnglish
Pages (from-to)575-579
Number of pages5
JournalHepatology
Volume25
Issue number3
DOIs
Publication statusPublished - 1997
Externally publishedYes

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Hepatocellular Carcinoma
Mutation
Messenger RNA
Cyclin-Dependent Kinases
Reverse Transcriptase Polymerase Chain Reaction
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Hepatology

Cite this

Reduced p21(WAF1/CIP1) expression and p53 mutation in hepatocellular carcinomas. / Hui, A. I Min; Kanai, Yae; Sakamoto, Michiie; Tsuda, Hitoshi; Hirohashi, Setsuo.

In: Hepatology, Vol. 25, No. 3, 1997, p. 575-579.

Research output: Contribution to journalArticle

Hui, A. I Min ; Kanai, Yae ; Sakamoto, Michiie ; Tsuda, Hitoshi ; Hirohashi, Setsuo. / Reduced p21(WAF1/CIP1) expression and p53 mutation in hepatocellular carcinomas. In: Hepatology. 1997 ; Vol. 25, No. 3. pp. 575-579.
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AU - Hirohashi, Setsuo

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