Reduced tumorigenicity and cell motility of a gastric carcinoma cell line by introduction of wild-type p53 gene

E. Kyo, H. Yokozaki, K. Yanagihara, W. Yasui, M. Ito, T. Sano, P. S. Lee, Hideyuki Saya, E. Tahara

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Wild-type or mutant human p53 gene was transfected into a human gastric carcinoma cell line MKN-1 which shares a mutant p53 allele. Transfected wild- type p53 reduced the colony forming efficiency and tumorigenicity of MKN-1 cells. However, no difference in expression of cell adhesion molecule, oncogenes and growth factors was observed among parent, wild-type p53 and mutant p53 transfectants. In motility assay, the wild-type p53 transfectants relative to the parental or mutant p53 transfectants exhibited a decreased motility, and HGF had a greater effect on the motility of the mutant p53 transfectants, but very little effect on the motility of either the parental or wild-type transfectants. In invasion assay, mutant p53 transfectants revealed the increased invasion ability into collagen gel. These results suggest that allele loss and point mutation of p53 gene may play a critical role not only in growth but also in invasion of gastric carcinoma cells.

Original languageEnglish
Pages (from-to)265-271
Number of pages7
JournalInternational Journal of Oncology
Volume3
Issue number2
Publication statusPublished - 1993
Externally publishedYes

Fingerprint

p53 Genes
Cell Movement
Stomach
Alleles
Carcinoma
Cell Line
Cell Adhesion Molecules
Oncogenes
Point Mutation
Intercellular Signaling Peptides and Proteins
Collagen
Gels
Growth
cell aggregation factors

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Kyo, E., Yokozaki, H., Yanagihara, K., Yasui, W., Ito, M., Sano, T., ... Tahara, E. (1993). Reduced tumorigenicity and cell motility of a gastric carcinoma cell line by introduction of wild-type p53 gene. International Journal of Oncology, 3(2), 265-271.

Reduced tumorigenicity and cell motility of a gastric carcinoma cell line by introduction of wild-type p53 gene. / Kyo, E.; Yokozaki, H.; Yanagihara, K.; Yasui, W.; Ito, M.; Sano, T.; Lee, P. S.; Saya, Hideyuki; Tahara, E.

In: International Journal of Oncology, Vol. 3, No. 2, 1993, p. 265-271.

Research output: Contribution to journalArticle

Kyo, E, Yokozaki, H, Yanagihara, K, Yasui, W, Ito, M, Sano, T, Lee, PS, Saya, H & Tahara, E 1993, 'Reduced tumorigenicity and cell motility of a gastric carcinoma cell line by introduction of wild-type p53 gene', International Journal of Oncology, vol. 3, no. 2, pp. 265-271.
Kyo, E. ; Yokozaki, H. ; Yanagihara, K. ; Yasui, W. ; Ito, M. ; Sano, T. ; Lee, P. S. ; Saya, Hideyuki ; Tahara, E. / Reduced tumorigenicity and cell motility of a gastric carcinoma cell line by introduction of wild-type p53 gene. In: International Journal of Oncology. 1993 ; Vol. 3, No. 2. pp. 265-271.
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