Reelin deficiency leads to aberrant lipid composition in mouse brain

Tomoharu Mizukami, Kazutaka Ikeda, Yuta Shimanaka, Katsunari Korogi, Chunyu Zhou, Hiroshi Takase, Hitomi Tsuiji, Nozomu Kono, Takao Kohno, Hiroyuki Arai, Makoto Arita, Mitsuharu Hattori

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Abstract

Reelin is a secreted protein essential for the development and function of the mammalian brain. The receptors for Reelin, apolipoprotein E receptor 2 and very low-density lipoprotein receptor, belong to the low-density lipoprotein receptor family, but it is not known whether Reelin is involved in the brain lipid metabolism. In the present study, we performed lipidomic analysis of the cerebral cortex of wild-type and Reelin-deficient (reeler) mice, and found that reeler mice exhibited several compositional changes in phospholipids. First, the ratio of phospholipids containing one saturated fatty acid (FA) and one docosahexaenoic acid (DHA) or arachidonic acid (ARA) decreased. Secondly, the ratio of phospholipids containing one monounsaturated FA (MUFA) and one DHA or ARA increased. Thirdly, the ratio of phospholipids containing 5,8,11-eicosatrienoic acid, or Mead acid (MA), increased. Finally, the expression of stearoyl-CoA desaturase-1 (SCD-1) increased. As the increase of MA is seen as an index of polyunsaturated FA (PUFA) deficiency, and the expression of SCD-1 is suppressed by PUFA, these results strongly suggest that the loss of Reelin leads to PUFA deficiency. Hence, MUFA and MA are synthesized in response to this deficiency, in part by inducing SCD-1 expression. This is the first report of changes of FA composition in the reeler mouse brain and provides a basis for further investigating the new role of Reelin in the development and function of the brain.

Original languageEnglish
Pages (from-to)81-86
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume505
Issue number1
DOIs
Publication statusPublished - 2018 Oct 20

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Stearoyl-CoA Desaturase
Brain
Phospholipids
Lipids
Docosahexaenoic Acids
Chemical analysis
Arachidonic Acid
Fatty Acids
Monounsaturated Fatty Acids
LDL Receptors
Unsaturated Fatty Acids
Lipid Metabolism
Cerebral Cortex
mead acid
Proteins

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Mizukami, T., Ikeda, K., Shimanaka, Y., Korogi, K., Zhou, C., Takase, H., ... Hattori, M. (2018). Reelin deficiency leads to aberrant lipid composition in mouse brain. Biochemical and Biophysical Research Communications, 505(1), 81-86. https://doi.org/10.1016/j.bbrc.2018.09.089

Reelin deficiency leads to aberrant lipid composition in mouse brain. / Mizukami, Tomoharu; Ikeda, Kazutaka; Shimanaka, Yuta; Korogi, Katsunari; Zhou, Chunyu; Takase, Hiroshi; Tsuiji, Hitomi; Kono, Nozomu; Kohno, Takao; Arai, Hiroyuki; Arita, Makoto; Hattori, Mitsuharu.

In: Biochemical and Biophysical Research Communications, Vol. 505, No. 1, 20.10.2018, p. 81-86.

Research output: Contribution to journalArticle

Mizukami, T, Ikeda, K, Shimanaka, Y, Korogi, K, Zhou, C, Takase, H, Tsuiji, H, Kono, N, Kohno, T, Arai, H, Arita, M & Hattori, M 2018, 'Reelin deficiency leads to aberrant lipid composition in mouse brain', Biochemical and Biophysical Research Communications, vol. 505, no. 1, pp. 81-86. https://doi.org/10.1016/j.bbrc.2018.09.089
Mizukami, Tomoharu ; Ikeda, Kazutaka ; Shimanaka, Yuta ; Korogi, Katsunari ; Zhou, Chunyu ; Takase, Hiroshi ; Tsuiji, Hitomi ; Kono, Nozomu ; Kohno, Takao ; Arai, Hiroyuki ; Arita, Makoto ; Hattori, Mitsuharu. / Reelin deficiency leads to aberrant lipid composition in mouse brain. In: Biochemical and Biophysical Research Communications. 2018 ; Vol. 505, No. 1. pp. 81-86.
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