Reelin is a secreted glycoprotein recognized by the CR-50 monoclonal antibody

Gabriella D'Arcangelo, Kazunori Nakajima, Takaki Miyata, Masaharu Ogawa, Katsuhiko Mikoshiba, Tom Curran

Research output: Contribution to journalArticle

417 Citations (Scopus)

Abstract

The neurological mouse mutant strain reeler displays abnormal laminar organization of several brain structures as a consequence of a defect in cell migration during neurodevelopment. This phenotype is a result of the disruption of reelin, a gene encoding a protein that has several structural characteristics of extracellular matrix proteins. To understand the molecular basis of the action of Reelin on neuronal migration, we constructed a full- length reelin clone and used it to direct Reelin expression. Here, we demonstrate that Reelin is a secreted glycoprotein and that a highly charged C-terminal region is essential for secretion. In addition, we demonstrate that an amino acid sequence present in the N-terminal region of Reelin contains an epitope that is recognized by the CR-50 monoclonal antibody. CR- 50 was raised against an antigen expressed in normal mouse brain that is absent in feeler mice. The interaction of CR-50 with its epitope leads to the disruption of neural cell aggregation in vitro. Here, we used CR-50 to precipitate Reelin from reticulocyte extracts programmed with reelin mRNA, from cells transfected with reelin clones, and from cerebellar explants. The reelin gene product seems to function as an instructive signal in the regulation of neuronal migration.

Original languageEnglish
Pages (from-to)23-31
Number of pages9
JournalJournal of Neuroscience
Volume17
Issue number1
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Epitopes
Glycoproteins
Mutant Strains Mice
Clone Cells
Monoclonal Antibodies
Neurologic Mutant Mice
Cell Aggregation
Extracellular Matrix Proteins
Reticulocytes
Brain
Genes
Cell Movement
Amino Acid Sequence
Phenotype
Antigens
Messenger RNA
Proteins
In Vitro Techniques

Keywords

  • cerebellum
  • cerebral cortex
  • extracellular matrix
  • glycosylation
  • mutant mice
  • neuronal migration
  • reeler

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

D'Arcangelo, G., Nakajima, K., Miyata, T., Ogawa, M., Mikoshiba, K., & Curran, T. (1997). Reelin is a secreted glycoprotein recognized by the CR-50 monoclonal antibody. Journal of Neuroscience, 17(1), 23-31.

Reelin is a secreted glycoprotein recognized by the CR-50 monoclonal antibody. / D'Arcangelo, Gabriella; Nakajima, Kazunori; Miyata, Takaki; Ogawa, Masaharu; Mikoshiba, Katsuhiko; Curran, Tom.

In: Journal of Neuroscience, Vol. 17, No. 1, 1997, p. 23-31.

Research output: Contribution to journalArticle

D'Arcangelo, G, Nakajima, K, Miyata, T, Ogawa, M, Mikoshiba, K & Curran, T 1997, 'Reelin is a secreted glycoprotein recognized by the CR-50 monoclonal antibody', Journal of Neuroscience, vol. 17, no. 1, pp. 23-31.
D'Arcangelo G, Nakajima K, Miyata T, Ogawa M, Mikoshiba K, Curran T. Reelin is a secreted glycoprotein recognized by the CR-50 monoclonal antibody. Journal of Neuroscience. 1997;17(1):23-31.
D'Arcangelo, Gabriella ; Nakajima, Kazunori ; Miyata, Takaki ; Ogawa, Masaharu ; Mikoshiba, Katsuhiko ; Curran, Tom. / Reelin is a secreted glycoprotein recognized by the CR-50 monoclonal antibody. In: Journal of Neuroscience. 1997 ; Vol. 17, No. 1. pp. 23-31.
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