Reelin receptors ApoER2 and VLDLR are expressed in distinct spatiotemporal patterns in developing mouse cerebral cortex

Yuki Hirota, Kenichiro Kubo, Kei ichi Katayama, Takao Honda, Takahiro Fujino, Tokuo T. Yamamoto, Kazunori Nakajima

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

In mammalian developing brain, neuronal migration is regulated by a variety of signaling cascades, including Reelin signaling. Reelin is a glycoprotein that is mainly secreted by Cajal-Retzius neurons in the marginal zone, playing essential roles in the formation of the layered neocortex via its receptors, apolipoprotein E receptor 2 (ApoER2) and very low density lipoprotein receptor (VLDLR). However, the precise mechanisms by which Reelin signaling controls the neuronal migration process remain unclear. To gain insight into how Reelin signaling controls individual migrating neurons, we generated monoclonal antibodies against ApoER2 and VLDLR and examined the localization of Reelin receptors in the developing mouse cerebral cortex. Immunohistochemical analyses revealed that VLDLR is localized to the distal portion of leading processes in the marginal zone (MZ), whereas ApoER2 is mainly localized to neuronal processes and the cell membranes of multipolar cells in the multipolar cell accumulation zone (MAZ). These different expression patterns may contribute to the distinct actions of Reelin on migrating neurons during both the early and late migratory stages in the developing cerebral cortex. J. Comp. Neurol. 523:463-478, 2015.

Original languageEnglish
Pages (from-to)463-478
Number of pages16
JournalJournal of Comparative Neurology
Volume523
Issue number3
DOIs
Publication statusPublished - 2015 Feb 15

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Cerebral Cortex
Neurons
Neocortex
Glycoproteins
Monoclonal Antibodies
Cell Membrane
Brain
reelin receptor
VLDL receptor
low density lipoprotein receptor-related protein 8

Keywords

  • ApoER2
  • Cerebral cortex
  • Migration
  • Reelin
  • RRID:AB_10862841
  • RRID:AB_2213003
  • RRID:AB_2253745
  • RRID:AB_2288612
  • RRID:AB_2336894
  • RRID:AB_2336895
  • RRID:AB_300798
  • RRID:AB_477577
  • RRID:AB_591819
  • RRID:AB_627679
  • RRID:AB_630921
  • RRID:IMSR_JAX:000235
  • RRID:IMSR_JAX:002529
  • VLDLR

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Reelin receptors ApoER2 and VLDLR are expressed in distinct spatiotemporal patterns in developing mouse cerebral cortex. / Hirota, Yuki; Kubo, Kenichiro; Katayama, Kei ichi; Honda, Takao; Fujino, Takahiro; Yamamoto, Tokuo T.; Nakajima, Kazunori.

In: Journal of Comparative Neurology, Vol. 523, No. 3, 15.02.2015, p. 463-478.

Research output: Contribution to journalArticle

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