Reeling-based cell fiber (Cell-F) assembly for the rapid construction of hierarchical tissues

Shintaroh Iwanaga, Hiroaki Onoe, Teru Okitsu, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This conference proceeding describes a rapid fabrication of hierarchical tissues with high cell density by cell fiber (Cell-F) assembling. We prepared cell-adhesive alginate fibers and cell-encapsulated fibers using with the same microfluidic device. Cells proliferated and connected within fibers, and cell fibers kept their fiber-shapes after removing alginate. Exposed cell fibers were reeled around cell-adhesive alginate (Alg-CoP) fiber with tweezers. These cell fibers easily adhered to Alg-CoP surfaces, and we succeeded in fabricating hierarchical thick tissues with 1×10<sup>8</sup> cells/cm<sup>3</sup> within 1 day. This approach would benefit for rapid fabrication of 3D tissue constructs with similar cell density to human body.

Original languageEnglish
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages1140-1141
Number of pages2
ISBN (Print)9780979806476
Publication statusPublished - 2014
Externally publishedYes
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: 2014 Oct 262014 Oct 30

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period14/10/2614/10/30

Keywords

  • Cell fiber
  • Hierarchical structure
  • Tissue engineering

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Iwanaga, S., Onoe, H., Okitsu, T., & Takeuchi, S. (2014). Reeling-based cell fiber (Cell-F) assembly for the rapid construction of hierarchical tissues. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 1140-1141). Chemical and Biological Microsystems Society.