Reference values for salivary cortisol in healthy young infants by liquid chromatography–tandem mass spectrometry

Mayako Saito-Abe, Kiwako Yamamoto-Hanada, Shoji F. Nakayama, Yuki Hashimoto, Osamu Natsume, Maki Fukami, Tomonobu Hasegawa, Yukihiro Ohya

Research output: Contribution to journalArticle

Abstract

Background: Sampling of salivary cortisol is non-invasive and important for the evaluation of the hypothalamic–pituitary–adrenal axis function and stress levels. However, the reference values for salivary cortisol measured by liquid chromatography–tandem mass spectrometry (LC-MS/MS) in healthy infants are unclear. The aim of this study was to establish the reference values for salivary cortisol levels in healthy infants. Methods: This study was a prospective observational cohort study following the participants until the age of 6 months. We analyzed 71 healthy, full-term infants at age 1 month between December 2017 and March 2018. We repeated saliva sampling every month, measured the salivary cortisol levels in the early morning by LC-MS/MS, and took the subjects’ medical history by questionnaire. Results: The minimum, 25th, 50th, 75th percentile, and maximum salivary cortisol levels were 0.08, 1.11, 2.21, 5.18, and 30.35 nmol/L, respectively. Conclusions: We established the reference values for salivary cortisol in young infants using LC-MS/MS.

Original languageEnglish
Pages (from-to)785-788
Number of pages4
JournalPediatrics International
Volume62
Issue number7
DOIs
Publication statusPublished - 2020 Jul 1

Keywords

  • LC-MS/MS
  • cortisol
  • infant
  • reference
  • saliva

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

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    Saito-Abe, M., Yamamoto-Hanada, K., Nakayama, S. F., Hashimoto, Y., Natsume, O., Fukami, M., Hasegawa, T., & Ohya, Y. (2020). Reference values for salivary cortisol in healthy young infants by liquid chromatography–tandem mass spectrometry. Pediatrics International, 62(7), 785-788. https://doi.org/10.1111/ped.14166