TY - JOUR
T1 - Reference values for salivary cortisol in healthy young infants by liquid chromatography–tandem mass spectrometry
AU - Saito-Abe, Mayako
AU - Yamamoto-Hanada, Kiwako
AU - Nakayama, Shoji F.
AU - Hashimoto, Yuki
AU - Natsume, Osamu
AU - Fukami, Maki
AU - Hasegawa, Tomonobu
AU - Ohya, Yukihiro
N1 - Funding Information:
We thank Mss Washizuka, Watabe, Hayase, and Monna for their contributions as research assistants. This study was funded by the Japan Agency for Medical Research and Development.
Publisher Copyright:
© 2020 Japan Pediatric Society
PY - 2020/7/1
Y1 - 2020/7/1
N2 - Background: Sampling of salivary cortisol is non-invasive and important for the evaluation of the hypothalamic–pituitary–adrenal axis function and stress levels. However, the reference values for salivary cortisol measured by liquid chromatography–tandem mass spectrometry (LC-MS/MS) in healthy infants are unclear. The aim of this study was to establish the reference values for salivary cortisol levels in healthy infants. Methods: This study was a prospective observational cohort study following the participants until the age of 6 months. We analyzed 71 healthy, full-term infants at age 1 month between December 2017 and March 2018. We repeated saliva sampling every month, measured the salivary cortisol levels in the early morning by LC-MS/MS, and took the subjects’ medical history by questionnaire. Results: The minimum, 25th, 50th, 75th percentile, and maximum salivary cortisol levels were 0.08, 1.11, 2.21, 5.18, and 30.35 nmol/L, respectively. Conclusions: We established the reference values for salivary cortisol in young infants using LC-MS/MS.
AB - Background: Sampling of salivary cortisol is non-invasive and important for the evaluation of the hypothalamic–pituitary–adrenal axis function and stress levels. However, the reference values for salivary cortisol measured by liquid chromatography–tandem mass spectrometry (LC-MS/MS) in healthy infants are unclear. The aim of this study was to establish the reference values for salivary cortisol levels in healthy infants. Methods: This study was a prospective observational cohort study following the participants until the age of 6 months. We analyzed 71 healthy, full-term infants at age 1 month between December 2017 and March 2018. We repeated saliva sampling every month, measured the salivary cortisol levels in the early morning by LC-MS/MS, and took the subjects’ medical history by questionnaire. Results: The minimum, 25th, 50th, 75th percentile, and maximum salivary cortisol levels were 0.08, 1.11, 2.21, 5.18, and 30.35 nmol/L, respectively. Conclusions: We established the reference values for salivary cortisol in young infants using LC-MS/MS.
KW - LC-MS/MS
KW - cortisol
KW - infant
KW - reference
KW - saliva
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U2 - 10.1111/ped.14166
DO - 10.1111/ped.14166
M3 - Article
C2 - 31976606
AN - SCOPUS:85087314749
VL - 62
SP - 785
EP - 788
JO - Pediatrics International
JF - Pediatrics International
SN - 1328-8067
IS - 7
ER -