Abstract
Urinary steroid profile analysis using gas chromatography/mass spectrometry (GC/MS) has been reported for the diagnosis of abnormal steroidogenesis in newborn infants with some success. We tried to establish the reference values of 63 urinary steroids in Japanese newborn infant, using GC/MS in selected ion monitoring (SIM) that utilizes two characteristic mass ions for each steroid for definitive identification. We studied 36 healthy full-term newborn infants (1-56 days of age) on spot urine samples to define the reference values (mg/g creatinine, median and 10-90 percentile range) and to investigate the possible difference between daytime and nighttime levels. We also studied 23 healthy adult females (20-24 years of age) on 24-hour-urine for the comparison of the reference values of newborn infants. Fifty metabolites of DHEA, pregnenolone, 17-hydroxypregnenolone, androstenedione, progesterone, 17-hydroxyprogesterone, 21-deoxycortisone, corticosterone, 18-hydroxycorticosterone, aldosterone, 18-hydroxycortisol, 11-deoxycortisol, cortisone, cortisol, and estrogen in each infant were measurable without interference, but 13 metabolites of 11-hydroxyandrostenedione, pregnenolone, 11-deoxycorticosterone, corticosterone, 11-dehydrocorticosterone, 21-deoxycortisol, 11-deoxycortisol and cortisol were unmeasurable in each infant due to the interference of fetal cortex steroids as confirmed by abnormal peak area ratios of two mass ions. All 63 metabolites in each control adult were measurable without interference. 16α-, 16β-, and 15β-hydroxy metabolites of 3β-hydroxy-5-en-steroids, and 6β-, 18-hydroxy and 11-oxo-metabolites of corticosteroids were significantly higher in full-term newborn infants than those in adults as previously reported. Urinary steroids showed little circadian variation in the newborn infants, indicating that spot urine can substitute for 24-hour urine.
| Original language | English |
|---|---|
| Pages (from-to) | 783-792 |
| Number of pages | 10 |
| Journal | Endocrine Journal |
| Volume | 50 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2003 Dec |
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Keywords
- GC/MS-SIM
- Newborn infant
- Profile analysis
- Reference values
- Steroidogenesis
- Urinary steroids
ASJC Scopus subject areas
- Endocrinology
Cite this
Reference Values for Urinary Steroids in Japanese Newborn Infants : Gas Chromatography/Mass Spectrometry in Selected Ion Monitoring. / Homma, Keiko; Hasegawa, Tomonobu; Masumoto, Masami; Takeshita, Eiko; Watanabe, Kiyoaki; Chiba, Hitoshi; Kurosawa, Takao; Takahashi, Takao; Matsuo, Nobutake.
In: Endocrine Journal, Vol. 50, No. 6, 12.2003, p. 783-792.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Reference Values for Urinary Steroids in Japanese Newborn Infants
T2 - Gas Chromatography/Mass Spectrometry in Selected Ion Monitoring
AU - Homma, Keiko
AU - Hasegawa, Tomonobu
AU - Masumoto, Masami
AU - Takeshita, Eiko
AU - Watanabe, Kiyoaki
AU - Chiba, Hitoshi
AU - Kurosawa, Takao
AU - Takahashi, Takao
AU - Matsuo, Nobutake
PY - 2003/12
Y1 - 2003/12
N2 - Urinary steroid profile analysis using gas chromatography/mass spectrometry (GC/MS) has been reported for the diagnosis of abnormal steroidogenesis in newborn infants with some success. We tried to establish the reference values of 63 urinary steroids in Japanese newborn infant, using GC/MS in selected ion monitoring (SIM) that utilizes two characteristic mass ions for each steroid for definitive identification. We studied 36 healthy full-term newborn infants (1-56 days of age) on spot urine samples to define the reference values (mg/g creatinine, median and 10-90 percentile range) and to investigate the possible difference between daytime and nighttime levels. We also studied 23 healthy adult females (20-24 years of age) on 24-hour-urine for the comparison of the reference values of newborn infants. Fifty metabolites of DHEA, pregnenolone, 17-hydroxypregnenolone, androstenedione, progesterone, 17-hydroxyprogesterone, 21-deoxycortisone, corticosterone, 18-hydroxycorticosterone, aldosterone, 18-hydroxycortisol, 11-deoxycortisol, cortisone, cortisol, and estrogen in each infant were measurable without interference, but 13 metabolites of 11-hydroxyandrostenedione, pregnenolone, 11-deoxycorticosterone, corticosterone, 11-dehydrocorticosterone, 21-deoxycortisol, 11-deoxycortisol and cortisol were unmeasurable in each infant due to the interference of fetal cortex steroids as confirmed by abnormal peak area ratios of two mass ions. All 63 metabolites in each control adult were measurable without interference. 16α-, 16β-, and 15β-hydroxy metabolites of 3β-hydroxy-5-en-steroids, and 6β-, 18-hydroxy and 11-oxo-metabolites of corticosteroids were significantly higher in full-term newborn infants than those in adults as previously reported. Urinary steroids showed little circadian variation in the newborn infants, indicating that spot urine can substitute for 24-hour urine.
AB - Urinary steroid profile analysis using gas chromatography/mass spectrometry (GC/MS) has been reported for the diagnosis of abnormal steroidogenesis in newborn infants with some success. We tried to establish the reference values of 63 urinary steroids in Japanese newborn infant, using GC/MS in selected ion monitoring (SIM) that utilizes two characteristic mass ions for each steroid for definitive identification. We studied 36 healthy full-term newborn infants (1-56 days of age) on spot urine samples to define the reference values (mg/g creatinine, median and 10-90 percentile range) and to investigate the possible difference between daytime and nighttime levels. We also studied 23 healthy adult females (20-24 years of age) on 24-hour-urine for the comparison of the reference values of newborn infants. Fifty metabolites of DHEA, pregnenolone, 17-hydroxypregnenolone, androstenedione, progesterone, 17-hydroxyprogesterone, 21-deoxycortisone, corticosterone, 18-hydroxycorticosterone, aldosterone, 18-hydroxycortisol, 11-deoxycortisol, cortisone, cortisol, and estrogen in each infant were measurable without interference, but 13 metabolites of 11-hydroxyandrostenedione, pregnenolone, 11-deoxycorticosterone, corticosterone, 11-dehydrocorticosterone, 21-deoxycortisol, 11-deoxycortisol and cortisol were unmeasurable in each infant due to the interference of fetal cortex steroids as confirmed by abnormal peak area ratios of two mass ions. All 63 metabolites in each control adult were measurable without interference. 16α-, 16β-, and 15β-hydroxy metabolites of 3β-hydroxy-5-en-steroids, and 6β-, 18-hydroxy and 11-oxo-metabolites of corticosteroids were significantly higher in full-term newborn infants than those in adults as previously reported. Urinary steroids showed little circadian variation in the newborn infants, indicating that spot urine can substitute for 24-hour urine.
KW - GC/MS-SIM
KW - Newborn infant
KW - Profile analysis
KW - Reference values
KW - Steroidogenesis
KW - Urinary steroids
UR - http://www.scopus.com/inward/record.url?scp=0942298678&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0942298678&partnerID=8YFLogxK
U2 - 10.1507/endocrj.50.783
DO - 10.1507/endocrj.50.783
M3 - Article
C2 - 14709852
AN - SCOPUS:0942298678
VL - 50
SP - 783
EP - 792
JO - Endocrine Journal
JF - Endocrine Journal
SN - 0918-8959
IS - 6
ER -