Refolding processes of cytochrome P450cam from ferric and ferrous acid forms to the native conformation. Formations of folding intermediates with non-native heme coordination state

Tsuyoshi Egawa, Takako Hishiki, Yusuke Ichikawa, Yasukazu Kanamori, Hideo Shimada, Satoshi Takahashi, Teizo Kitagawa, Yuzuru Ishimura

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Abstract

Changes in heme coordination state and protein conformation of cytochrome P450cam (P450cam), a b-type heme protein, were investigated by employing pH jump experiments coupled with time-resolved optical absorption, fluorescence, circular dichroism, and resonance Raman techniques. We found a partially unfolded form (acid form) of ferric P450cam at pH 2.5, in which a Cys-heme coordination bond in the native conformation was ruptured. When the pH was raised to pH 7.5, the acid form refolded to the native conformation through a distinctive intermediate. Formations of similar acid and intermediate forms were also observed for ferrous P450cam. Both the ferric and ferrous forms of the intermediate were found to have an unidentified axial ligand of the heme at the 6th coordination sphere, which is vacant in the high spin ferric and ferrous forms at the native conformation. For the ferrous form, it was also indicated that the 5th axial ligand is different from the native cysteinate. The folding intermediates identified in this study demonstrate occurrences of non-native coordination state of heme during the refolding processes of the large b-type heme protein, being akin to the well known folding intermediates of cytochromes c, in which c-type heme is covalently attached to a smaller protein.

Original languageEnglish
Pages (from-to)32008-32017
Number of pages10
JournalJournal of Biological Chemistry
Volume279
Issue number31
DOIs
Publication statusPublished - 2004 Jul 30

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Camphor 5-Monooxygenase
Cytochromes
Heme
Conformations
Acids
Hemeproteins
Ligands
Protein Conformation
Circular Dichroism
Cytochromes c
Light absorption
Proteins
Fluorescence

ASJC Scopus subject areas

  • Biochemistry

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Refolding processes of cytochrome P450cam from ferric and ferrous acid forms to the native conformation. Formations of folding intermediates with non-native heme coordination state. / Egawa, Tsuyoshi; Hishiki, Takako; Ichikawa, Yusuke; Kanamori, Yasukazu; Shimada, Hideo; Takahashi, Satoshi; Kitagawa, Teizo; Ishimura, Yuzuru.

In: Journal of Biological Chemistry, Vol. 279, No. 31, 30.07.2004, p. 32008-32017.

Research output: Contribution to journalArticle

Egawa, Tsuyoshi ; Hishiki, Takako ; Ichikawa, Yusuke ; Kanamori, Yasukazu ; Shimada, Hideo ; Takahashi, Satoshi ; Kitagawa, Teizo ; Ishimura, Yuzuru. / Refolding processes of cytochrome P450cam from ferric and ferrous acid forms to the native conformation. Formations of folding intermediates with non-native heme coordination state. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 31. pp. 32008-32017.
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abstract = "Changes in heme coordination state and protein conformation of cytochrome P450cam (P450cam), a b-type heme protein, were investigated by employing pH jump experiments coupled with time-resolved optical absorption, fluorescence, circular dichroism, and resonance Raman techniques. We found a partially unfolded form (acid form) of ferric P450cam at pH 2.5, in which a Cys-heme coordination bond in the native conformation was ruptured. When the pH was raised to pH 7.5, the acid form refolded to the native conformation through a distinctive intermediate. Formations of similar acid and intermediate forms were also observed for ferrous P450cam. Both the ferric and ferrous forms of the intermediate were found to have an unidentified axial ligand of the heme at the 6th coordination sphere, which is vacant in the high spin ferric and ferrous forms at the native conformation. For the ferrous form, it was also indicated that the 5th axial ligand is different from the native cysteinate. The folding intermediates identified in this study demonstrate occurrences of non-native coordination state of heme during the refolding processes of the large b-type heme protein, being akin to the well known folding intermediates of cytochromes c, in which c-type heme is covalently attached to a smaller protein.",
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T1 - Refolding processes of cytochrome P450cam from ferric and ferrous acid forms to the native conformation. Formations of folding intermediates with non-native heme coordination state

AU - Egawa, Tsuyoshi

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AU - Ichikawa, Yusuke

AU - Kanamori, Yasukazu

AU - Shimada, Hideo

AU - Takahashi, Satoshi

AU - Kitagawa, Teizo

AU - Ishimura, Yuzuru

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N2 - Changes in heme coordination state and protein conformation of cytochrome P450cam (P450cam), a b-type heme protein, were investigated by employing pH jump experiments coupled with time-resolved optical absorption, fluorescence, circular dichroism, and resonance Raman techniques. We found a partially unfolded form (acid form) of ferric P450cam at pH 2.5, in which a Cys-heme coordination bond in the native conformation was ruptured. When the pH was raised to pH 7.5, the acid form refolded to the native conformation through a distinctive intermediate. Formations of similar acid and intermediate forms were also observed for ferrous P450cam. Both the ferric and ferrous forms of the intermediate were found to have an unidentified axial ligand of the heme at the 6th coordination sphere, which is vacant in the high spin ferric and ferrous forms at the native conformation. For the ferrous form, it was also indicated that the 5th axial ligand is different from the native cysteinate. The folding intermediates identified in this study demonstrate occurrences of non-native coordination state of heme during the refolding processes of the large b-type heme protein, being akin to the well known folding intermediates of cytochromes c, in which c-type heme is covalently attached to a smaller protein.

AB - Changes in heme coordination state and protein conformation of cytochrome P450cam (P450cam), a b-type heme protein, were investigated by employing pH jump experiments coupled with time-resolved optical absorption, fluorescence, circular dichroism, and resonance Raman techniques. We found a partially unfolded form (acid form) of ferric P450cam at pH 2.5, in which a Cys-heme coordination bond in the native conformation was ruptured. When the pH was raised to pH 7.5, the acid form refolded to the native conformation through a distinctive intermediate. Formations of similar acid and intermediate forms were also observed for ferrous P450cam. Both the ferric and ferrous forms of the intermediate were found to have an unidentified axial ligand of the heme at the 6th coordination sphere, which is vacant in the high spin ferric and ferrous forms at the native conformation. For the ferrous form, it was also indicated that the 5th axial ligand is different from the native cysteinate. The folding intermediates identified in this study demonstrate occurrences of non-native coordination state of heme during the refolding processes of the large b-type heme protein, being akin to the well known folding intermediates of cytochromes c, in which c-type heme is covalently attached to a smaller protein.

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