Refractive index difference sensing illuminated by evanescent wave for noninvasive diagnosis of cell's pathological state

Reiko Kuiryama, Yuta Tanaka, Yohei Sato

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study reports the noninvasive, wide-field and rapid measurement of refractive index (RI) difference for diagnosis of cell's pathological state by utilizing light scattering with evanescent wave illumination. The RI of an abnormal cell is different from that of a normal one because of the change in the ratio of each intracellular component, thus RI measurement can be an effective approach for diagnosing cells. The present study focuses on the development of noninvasive RI difference measurement technique which can be applied to various biomedical analyses in the field of tissue engineering or drug discovery. When cells on a glass substrate are illuminated by the evanescent wave, scattered light whose intensity depends on the RI difference is generated at the interface between each cell and solution. In order to validate the effectiveness of this measurement method, the present technique was applied to the interface between PDMS and solution in the microchannel. The quantitative relationship between the RI difference and the scattered light intensity was obtained by adjusting the RI difference at the interface. It can be concluded that this measurement technique has possibilities to distinguish abnormal cells from normal ones by the scattered light intensity.

Original languageEnglish
Title of host publication2010 International Symposium on Micro-NanoMechatronics and Human Science
Subtitle of host publicationFrom Micro and Nano Scale Systems to Robotics and Mechatronics Systems, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010
Pages7-10
Number of pages4
DOIs
Publication statusPublished - 2010 Dec 1
Event21st Annual Symposium on Micro-Nano Mechatronics and Human Science, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010 - Nagoya, Japan
Duration: 2010 Nov 72010 Nov 10

Publication series

Name2010 International Symposium on Micro-NanoMechatronics and Human Science: From Micro and Nano Scale Systems to Robotics and Mechatronics Systems, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010

Other

Other21st Annual Symposium on Micro-Nano Mechatronics and Human Science, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010
CountryJapan
CityNagoya
Period10/11/710/11/10

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering

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    Kuiryama, R., Tanaka, Y., & Sato, Y. (2010). Refractive index difference sensing illuminated by evanescent wave for noninvasive diagnosis of cell's pathological state. In 2010 International Symposium on Micro-NanoMechatronics and Human Science: From Micro and Nano Scale Systems to Robotics and Mechatronics Systems, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010 (pp. 7-10). [5669585] (2010 International Symposium on Micro-NanoMechatronics and Human Science: From Micro and Nano Scale Systems to Robotics and Mechatronics Systems, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010). https://doi.org/10.1109/MHS.2010.5669585