Regenerative Growth in Drosophila Imaginal Discs Is Regulated by Wingless and Myc

Rachel K. Smith-Bolton, Melanie I. Worley, Hiroshi Kanda, Iswar K. Hariharan

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

The study of regeneration would be aided greatly by systems that support large-scale genetic screens. Here we describe a nonsurgical method for inducing tissue damage and regeneration in Drosophila larvae by inducing apoptosis in the wing imaginal disc in a spatially and temporally regulated manner. Tissue damage results in localized regenerative proliferation characterized by altered expression of patterning genes and growth regulators as well as a temporary loss of markers of cell fate commitment. Wingless and Myc are induced by tissue damage and are important for regenerative growth. Furthermore, ectopic Myc enhances regeneration when other growth drivers tested do not. As the animal matures, the ability to regenerate is lost and cannot be restored by activation of Wingless or Myc. This system is conducive to forward genetic screens, enabling an unbiased search for genes that regulate both the extent of and the capacity for regeneration.

Original languageEnglish
Pages (from-to)797-809
Number of pages13
JournalDevelopmental Cell
Volume16
Issue number6
DOIs
Publication statusPublished - 2009 Jun 16
Externally publishedYes

Fingerprint

Imaginal Discs
Drosophila
Regeneration
Tissue
Growth
Genes
Animals
Regulator Genes
Chemical activation
Apoptosis
Larva
Gene Expression

Keywords

  • DEVBIO

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Regenerative Growth in Drosophila Imaginal Discs Is Regulated by Wingless and Myc. / Smith-Bolton, Rachel K.; Worley, Melanie I.; Kanda, Hiroshi; Hariharan, Iswar K.

In: Developmental Cell, Vol. 16, No. 6, 16.06.2009, p. 797-809.

Research output: Contribution to journalArticle

Smith-Bolton, Rachel K. ; Worley, Melanie I. ; Kanda, Hiroshi ; Hariharan, Iswar K. / Regenerative Growth in Drosophila Imaginal Discs Is Regulated by Wingless and Myc. In: Developmental Cell. 2009 ; Vol. 16, No. 6. pp. 797-809.
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