Regulation of adult neural progenitor cells by Galectin-1/β1 Integrin interaction

Masanori Sakaguchi, Yoichi Imaizumi, Tetsuro Shingo, Hirobumi Tada, Ko Hayama, Osamu Yamada, Tsuyoshi Morishita, Toshihiko Kadoya, Noboru Uchiyama, Takuya Shimazaki, Atsushi Kuno, Françoise Poirier, Jun Hirabayashi, Kazunobu Sawamoto, Hideyuki Okano

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Neural stem cells (NSCs) proliferate and generate new neurons in the adult brain. A carbohydrate-binding protein (lectin), Galectin-1, is expressed in the NSCs in the subependymal zone (SEZ) of the adult mouse brain. The infusion and knockout of Galectin-1 in the SEZ results in an increase and decrease, respectively, of NSCs and subsequently born progenitor cells. The molecular mechanism of this effect, however, has been unknown. Previous studies outside the brain suggest that Galectin-1 binds to a carbohydrate structure of β1 Integrin and modulates cell adhesion. Here, we studied the functional interaction between Galectin-1 and β1 Integrin in the adult mouse SEZ. β1 Integrin was purified from adult SEZ tissue by binding to a Galectin-1 affinity column, and this binding depended on Galectin-1's carbohydrate-binding activity. In adult brain sections, Galectin-1-binding activity was detected on β1 Integrin-expressing cells in the SEZ. Furthermore, in the adult SEZ, the simultaneous infusion of a β1 Integrin-neutralizing antibody with Galectin-1 protein reversed the increasing effect of Galectin-1 on the number of adult neural progenitor cells (NPCs). Finally, intact β1 Integrin was required for Galectin-1's function in NPC adhesion in vitro. These results suggest that the interaction between β1 Integrin and Galectin-1 plays an important role in regulating the number of adult NPCs through mechanisms including cell adhesion.

Original languageEnglish
Pages (from-to)1516-1524
Number of pages9
JournalJournal of Neurochemistry
Volume113
Issue number6
DOIs
Publication statusPublished - 2010 Jun

Fingerprint

Galectin 1
Integrins
Stem Cells
Neural Stem Cells
Brain
Cell adhesion
Stem cells
Cell Adhesion
Carbohydrates
Neutralizing Antibodies
Lectins
Neurons

Keywords

  • β1 Integrin
  • Adult neural progenitor cells
  • Galectin-1
  • Lectin

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Regulation of adult neural progenitor cells by Galectin-1/β1 Integrin interaction. / Sakaguchi, Masanori; Imaizumi, Yoichi; Shingo, Tetsuro; Tada, Hirobumi; Hayama, Ko; Yamada, Osamu; Morishita, Tsuyoshi; Kadoya, Toshihiko; Uchiyama, Noboru; Shimazaki, Takuya; Kuno, Atsushi; Poirier, Françoise; Hirabayashi, Jun; Sawamoto, Kazunobu; Okano, Hideyuki.

In: Journal of Neurochemistry, Vol. 113, No. 6, 06.2010, p. 1516-1524.

Research output: Contribution to journalArticle

Sakaguchi, M, Imaizumi, Y, Shingo, T, Tada, H, Hayama, K, Yamada, O, Morishita, T, Kadoya, T, Uchiyama, N, Shimazaki, T, Kuno, A, Poirier, F, Hirabayashi, J, Sawamoto, K & Okano, H 2010, 'Regulation of adult neural progenitor cells by Galectin-1/β1 Integrin interaction', Journal of Neurochemistry, vol. 113, no. 6, pp. 1516-1524. https://doi.org/10.1111/j.1471-4159.2010.06712.x
Sakaguchi, Masanori ; Imaizumi, Yoichi ; Shingo, Tetsuro ; Tada, Hirobumi ; Hayama, Ko ; Yamada, Osamu ; Morishita, Tsuyoshi ; Kadoya, Toshihiko ; Uchiyama, Noboru ; Shimazaki, Takuya ; Kuno, Atsushi ; Poirier, Françoise ; Hirabayashi, Jun ; Sawamoto, Kazunobu ; Okano, Hideyuki. / Regulation of adult neural progenitor cells by Galectin-1/β1 Integrin interaction. In: Journal of Neurochemistry. 2010 ; Vol. 113, No. 6. pp. 1516-1524.
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AU - Morishita, Tsuyoshi

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