Regulation of ascidian Rel by its alternative splice variant

Narudo Kawai, Masumi Shimada, Hiroyuki Kawahara, Noriyuki Satoh, Hideyoshi Yokosawa

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The Rel/NF-κB family of transcription factors play key roles in morphogenesis and immune responses. We reported previously that As-rel1 and As-rel2 of the ascidian Halocynthia roretzi are involved in notochord formation. The As-rel1 protein is a typical Rel/NF-κB family member, whereas the As-rel2 protein is a novel truncated product of As-rel1 that lacks a nuclear localization signal and the unique C-terminal region. Here, we present conclusive evidence that As-rel1 and As-rel2 are generated from a single gene by alternative splicing. We analyzed the roles of As-rel2 using cells transfected with As-rel1 or As-rel2 or both. As-rel1 was localized in the nucleus and As-rel2 in the cytoplasm when they were transfected individually. In contrast, when they were transfected simultaneously, both were localized in the nucleus because of the association of As-rel2 with As-rel1. In this case, the transcriptional activity of As-rel1 was suppressed by As-rel2. Ascidian IκB was found to sequester As-rel1 in the cytoplasm and suppress its transcriptional activity when As-rel1 and IκB were transfected simultaneously. In contrast, when As-rel1 and IκB were transfected together with As-rel2, As-rel1 was transported into the nucleus and its transcriptional activity was rescued from inhibition by IκB, whereas As-rel2 remained localized in the cytoplasm, suggesting IκB sequestration in the cytoplasm by As-rel2. From these findings, we conclude that the alternative splice variant, As-rel2, regulates the nuclear localization and transcriptional activity of As-rel1.

Original languageEnglish
Pages (from-to)4459-4468
Number of pages10
JournalEuropean Journal of Biochemistry
Volume270
Issue number22
DOIs
Publication statusPublished - 2003 Nov
Externally publishedYes

Fingerprint

Urochordata
Cytoplasm
Alternative Splicing
Proteins
Transcription Factors
Genes
Notochord
Nuclear Localization Signals
Morphogenesis

Keywords

  • Alternative splicing
  • IκB
  • Nuclear transport
  • Rel
  • Transcriptional activity

ASJC Scopus subject areas

  • Biochemistry

Cite this

Kawai, N., Shimada, M., Kawahara, H., Satoh, N., & Yokosawa, H. (2003). Regulation of ascidian Rel by its alternative splice variant. European Journal of Biochemistry, 270(22), 4459-4468. https://doi.org/10.1046/j.1432-1033.2003.03838.x

Regulation of ascidian Rel by its alternative splice variant. / Kawai, Narudo; Shimada, Masumi; Kawahara, Hiroyuki; Satoh, Noriyuki; Yokosawa, Hideyoshi.

In: European Journal of Biochemistry, Vol. 270, No. 22, 11.2003, p. 4459-4468.

Research output: Contribution to journalArticle

Kawai, N, Shimada, M, Kawahara, H, Satoh, N & Yokosawa, H 2003, 'Regulation of ascidian Rel by its alternative splice variant', European Journal of Biochemistry, vol. 270, no. 22, pp. 4459-4468. https://doi.org/10.1046/j.1432-1033.2003.03838.x
Kawai, Narudo ; Shimada, Masumi ; Kawahara, Hiroyuki ; Satoh, Noriyuki ; Yokosawa, Hideyoshi. / Regulation of ascidian Rel by its alternative splice variant. In: European Journal of Biochemistry. 2003 ; Vol. 270, No. 22. pp. 4459-4468.
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