Abstract
The Drosophila strawberry (sty) locus was isolated by P-element insertion mutagenesis in a screen for mutations affecting eye development. Analysis of the mutant phenotype and the putative expression pattern of the sty gene suggested that it has multiple functions. Mutations in the sty gene lead to irregular spacing of ommatidia, an increase in the number of photoreceptor cells, as well as abnormal axonal projections to the lamina and disrupted structure of the optic lobes in the adult fly. The sty mutation also causes abnormal head involution, a change in a number of sensilla in the antennomaxillary complex in the embryonic stage and abnormal morphogenesis of the maxillary palp and wings in later stages. We examined the presumptive expression of the sty gene during development by histochemical staining for lacZ expression from enhancer trap elements inserted within the sty gene. During embryogenesis, expression of lacZ showed a segmental pattern in the ectoderm and in the nervous system. In the eye imaginal discs, lacZ was expressed in photoreceptor cells beginning a few rows posterior to the morphogenetic furrow. The lacZ was also expressed in the wing disc. In the adult, lacZ was expressed in the retina and lamina. We cloned the sty gene by P-element tagging and found that it encodes a putative secreted protein containing a cysteine-rich region similar to the epidermal growth factor (EGF) repeat. On the basis of the loss of function phenotype, the expression pattern and the predicted structure of its product, we propose that sty encodes a diffusible protein acting as a signal involved in lateral inhibition within the developing nervous system and also as a factor involved either directly or indirectly in axonal guidance and optic lobe development.
| Original language | English |
|---|---|
| Pages (from-to) | 1-11 |
| Number of pages | 11 |
| Journal | Differentiation |
| Volume | 52 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1993 |
| Externally published | Yes |
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Keywords
- Argos
- Drosophila
- Eye development
- Giant lens
- Neuronal cell fates
- Secreted protein
- Strawberry
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Developmental Biology
- Cell Biology
Cite this
Regulation of Drosophila neural development by a putative secreted protein. / Okano, Hideyuki; Hayashi, S.; Tanimura, T.; Sawamoto, K.; Yoshikawa, S.; Watanabe, J.; Iwasaki, M.; Hirose, S.; Mikoshiba, K.; Montell, C.
In: Differentiation, Vol. 52, No. 1, 1993, p. 1-11.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Regulation of Drosophila neural development by a putative secreted protein
AU - Okano, Hideyuki
AU - Hayashi, S.
AU - Tanimura, T.
AU - Sawamoto, K.
AU - Yoshikawa, S.
AU - Watanabe, J.
AU - Iwasaki, M.
AU - Hirose, S.
AU - Mikoshiba, K.
AU - Montell, C.
PY - 1993
Y1 - 1993
N2 - The Drosophila strawberry (sty) locus was isolated by P-element insertion mutagenesis in a screen for mutations affecting eye development. Analysis of the mutant phenotype and the putative expression pattern of the sty gene suggested that it has multiple functions. Mutations in the sty gene lead to irregular spacing of ommatidia, an increase in the number of photoreceptor cells, as well as abnormal axonal projections to the lamina and disrupted structure of the optic lobes in the adult fly. The sty mutation also causes abnormal head involution, a change in a number of sensilla in the antennomaxillary complex in the embryonic stage and abnormal morphogenesis of the maxillary palp and wings in later stages. We examined the presumptive expression of the sty gene during development by histochemical staining for lacZ expression from enhancer trap elements inserted within the sty gene. During embryogenesis, expression of lacZ showed a segmental pattern in the ectoderm and in the nervous system. In the eye imaginal discs, lacZ was expressed in photoreceptor cells beginning a few rows posterior to the morphogenetic furrow. The lacZ was also expressed in the wing disc. In the adult, lacZ was expressed in the retina and lamina. We cloned the sty gene by P-element tagging and found that it encodes a putative secreted protein containing a cysteine-rich region similar to the epidermal growth factor (EGF) repeat. On the basis of the loss of function phenotype, the expression pattern and the predicted structure of its product, we propose that sty encodes a diffusible protein acting as a signal involved in lateral inhibition within the developing nervous system and also as a factor involved either directly or indirectly in axonal guidance and optic lobe development.
AB - The Drosophila strawberry (sty) locus was isolated by P-element insertion mutagenesis in a screen for mutations affecting eye development. Analysis of the mutant phenotype and the putative expression pattern of the sty gene suggested that it has multiple functions. Mutations in the sty gene lead to irregular spacing of ommatidia, an increase in the number of photoreceptor cells, as well as abnormal axonal projections to the lamina and disrupted structure of the optic lobes in the adult fly. The sty mutation also causes abnormal head involution, a change in a number of sensilla in the antennomaxillary complex in the embryonic stage and abnormal morphogenesis of the maxillary palp and wings in later stages. We examined the presumptive expression of the sty gene during development by histochemical staining for lacZ expression from enhancer trap elements inserted within the sty gene. During embryogenesis, expression of lacZ showed a segmental pattern in the ectoderm and in the nervous system. In the eye imaginal discs, lacZ was expressed in photoreceptor cells beginning a few rows posterior to the morphogenetic furrow. The lacZ was also expressed in the wing disc. In the adult, lacZ was expressed in the retina and lamina. We cloned the sty gene by P-element tagging and found that it encodes a putative secreted protein containing a cysteine-rich region similar to the epidermal growth factor (EGF) repeat. On the basis of the loss of function phenotype, the expression pattern and the predicted structure of its product, we propose that sty encodes a diffusible protein acting as a signal involved in lateral inhibition within the developing nervous system and also as a factor involved either directly or indirectly in axonal guidance and optic lobe development.
KW - Argos
KW - Drosophila
KW - Eye development
KW - Giant lens
KW - Neuronal cell fates
KW - Secreted protein
KW - Strawberry
UR - http://www.scopus.com/inward/record.url?scp=0027511739&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027511739&partnerID=8YFLogxK
U2 - 10.1111/j.1432-0436.1992.tb00494.x
DO - 10.1111/j.1432-0436.1992.tb00494.x
M3 - Article
C2 - 1286772
AN - SCOPUS:0027511739
VL - 52
SP - 1
EP - 11
JO - Differentiation
JF - Differentiation
SN - 0301-4681
IS - 1
ER -