Regulation of expression of the galactose gene cluster in Saccharomyces cerevisiae - II. The isolation and dosage effect of the regulatory gene GAL80

Yasuhisa Nogi, Hideo Shimada, Yuriko Matsuzaki, Hideaki Hashimoto, Toshio Fukasawa

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The galactose analogue 2-deoxygalactose was found to inhibit the growth of a mutant strain of Saccharomyces cerevisiae constitutively producing the set of galactose utilization enzymes. Based on this fact, the yeast GAL80 gene negatively regulating the expression of the genes encoding those enzymes was isolated for its ability to confer 2-deoxygalactose resistance on a strain carrying a recessive mutation in that gene. The GAL80 gene was located within a 3.0 kb fragment in the cloned DNA. When the isolated gene was incorporated into a multi-copy plasmid, the induced level of three enzymes encoded by the gene cluster GAL7-GAL10-GAL1 in the host chromosome was lowered. Such a gene dosage effect of GAL80 was further pronounced if sucrose, a sugar causing catabolite repression, was added to the growth medium. The ratio of the enzyme activity of the yeast bearing multiple copies of GAL80 to that of the yeast bearing its single copy significantly varied with the enzyme. From these results we suggest that the intracellular inducer interacts with the GAL80 product and that GAL80 molecules directly bind the GAL cluster genes with an affinity different from one gene to another.

Original languageEnglish
Pages (from-to)29-34
Number of pages6
JournalMGG Molecular & General Genetics
Volume195
Issue number1-2
DOIs
Publication statusPublished - 1984 Jun

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Regulator Genes
Multigene Family
Galactose
Saccharomyces cerevisiae
Enzymes
Fucose
Yeasts
Genes
Catabolite Repression
Gene Dosage
Growth
Sucrose
Plasmids
Chromosomes
Gene Expression
Mutation
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Regulation of expression of the galactose gene cluster in Saccharomyces cerevisiae - II. The isolation and dosage effect of the regulatory gene GAL80. / Nogi, Yasuhisa; Shimada, Hideo; Matsuzaki, Yuriko; Hashimoto, Hideaki; Fukasawa, Toshio.

In: MGG Molecular & General Genetics, Vol. 195, No. 1-2, 06.1984, p. 29-34.

Research output: Contribution to journalArticle

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