Regulation of FcεRI-mediated signaling by an adaptor protein STAP-2/BSK in rat basophilic leukemia RBL-2H3 cells

Tetsuya Yamamoto, Taro Yumioka, Yuichi Sekine, Noriko Sato, Mayu Minoguchi, Akihiko Yoshimura, Tadashi Matsuda

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Crosslinking of multivalent antigen bound IgE transduces FcεRI mediated signaling cascades, which activate nonreceptor-type protein-tyrosine kinases and subsequent tyrosine phosphorylation of cellular proteins, and these are critical elements for degranulation in mast cells. We cloned a novel adaptor molecule, signal transducing adaptor protein (STAP)-2 containing PH and SH2-like domains as a c-fms interacting protein. STAP-2 was identical to a recently cloned adaptor molecule, BKS, a substrate of BRK (breast tumor kinase) tyrosine kinase, although its function is still unknown. To examine a novel function of STAP-2/BSK, we expressed STAP-2/BSK or its mutants in rat basophilic leukemia RBL-2H3 cells. Overexpression of STAP-2/BSK resulted in a suppression of FcεRI-mediated calcium mobilization and degranulation. FcεRI-induced tyrosine phosphorylation of phospholipase C-γ (PLC-γ) but not Syk was significantly suppressed in these cells. Furthermore, STAP-2/BSK associated with PLC-γ in vivo. These data indicate that STAP-2/BSK negatively controls the FcεRI-mediated calcium mobilization and degranulation by direct modulation of tyrosine phosphorylation of PLC-γ.

Original languageEnglish
Pages (from-to)767-773
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume306
Issue number3
DOIs
Publication statusPublished - 2003 Jul 4
Externally publishedYes

Fingerprint

Signal Transducing Adaptor Proteins
Rats
Leukemia
Proteins
Phosphorylation
Type C Phospholipases
Tyrosine
Protein-Tyrosine Kinases
Macrophage Colony-Stimulating Factor Receptors
Calcium
Molecules
src Homology Domains
Mast Cells
Crosslinking
Immunoglobulin E
Tumors
Phosphotransferases
Modulation

Keywords

  • High affinity receptor for IgE
  • Phospholipase C-γ
  • Signal transducing adaptor protein-2

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Regulation of FcεRI-mediated signaling by an adaptor protein STAP-2/BSK in rat basophilic leukemia RBL-2H3 cells. / Yamamoto, Tetsuya; Yumioka, Taro; Sekine, Yuichi; Sato, Noriko; Minoguchi, Mayu; Yoshimura, Akihiko; Matsuda, Tadashi.

In: Biochemical and Biophysical Research Communications, Vol. 306, No. 3, 04.07.2003, p. 767-773.

Research output: Contribution to journalArticle

Yamamoto, Tetsuya ; Yumioka, Taro ; Sekine, Yuichi ; Sato, Noriko ; Minoguchi, Mayu ; Yoshimura, Akihiko ; Matsuda, Tadashi. / Regulation of FcεRI-mediated signaling by an adaptor protein STAP-2/BSK in rat basophilic leukemia RBL-2H3 cells. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 306, No. 3. pp. 767-773.
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