Regulation of FGF receptor-2 expression by transcription factor E2F-1

Etsu Tashiro, Yusuke Minato, Hiroko Maruki, Masataka Asagiri, Masaya Imoto

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Fibroblast growth factors (FGF) and their receptors play an important role in cell proliferation, angiogenesis and embryonal development. In this study, we show that expression of the FGF receptor-2 (FGFR-2) protein is induced in the mid-to-late G1 phase of the cell cycle in serum-starved mouse NIH3T3 cells released from starvation. Transcription of mouse FGFR-2 was activated by E2F-1. Analysis of various mouse FGFR-2 promoter mutant constructs showed that a sequence located +57/+64 downstream of the transcriptional initiation site, related to the consensus E2F-responsive sequence, is necessary for the activation. The promoter activity of the mouse FGFR-2 gene is also positively regulated by E2F-2 and E2F-3, but not by E2F-4 and E2F-5. Moreover, the E2F-1-induced activation of mouse FGFR-2 gene transcription is suppressed by pRB. Taken together, the results demonstrate that FGFR-2 is a new class of targets for E2F, and expression of mouse FGFR-2 in mid-to-late G1 phase would be mediated, at least in part, by the activation of a pRB/E2F pathway.

Original languageEnglish
Pages (from-to)5630-5635
Number of pages6
JournalOncogene
Volume22
Issue number36
DOIs
Publication statusPublished - 2003 Jan 1

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Keywords

  • Cell cycle
  • E2F-1
  • FGFR-2
  • Promoter
  • Transcription
  • pRB

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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