Abstract
Fibroblast growth factors (FGF) and their receptors play an important role in cell proliferation, angiogenesis and embryonal development. In this study, we show that expression of the FGF receptor-2 (FGFR-2) protein is induced in the mid-to-late G1 phase of the cell cycle in serum-starved mouse NIH3T3 cells released from starvation. Transcription of mouse FGFR-2 was activated by E2F-1. Analysis of various mouse FGFR-2 promoter mutant constructs showed that a sequence located +57/+64 downstream of the transcriptional initiation site, related to the consensus E2F-responsive sequence, is necessary for the activation. The promoter activity of the mouse FGFR-2 gene is also positively regulated by E2F-2 and E2F-3, but not by E2F-4 and E2F-5. Moreover, the E2F-1-induced activation of mouse FGFR-2 gene transcription is suppressed by pRB. Taken together, the results demonstrate that FGFR-2 is a new class of targets for E2F, and expression of mouse FGFR-2 in mid-to-late G1 phase would be mediated, at least in part, by the activation of a pRB/E2F pathway.
Original language | English |
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Pages (from-to) | 5630-5635 |
Number of pages | 6 |
Journal | Oncogene |
Volume | 22 |
Issue number | 36 |
DOIs | |
Publication status | Published - 2003 |
Externally published | Yes |
Keywords
- Cell cycle
- E2F-1
- FGFR-2
- Promoter
- Transcription
- pRB
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Cancer Research