Regulation of granulocyte colony-stimulating factor receptor-mediated granulocytic differentiation by C-mannosylation

Kei Otani, Yuki Niwa, Takehiro Suzuki, Natsumi Sato, Yukiko Sasazawa, Naoshi Dohmae, Siro Simizu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Granulocyte colony-stimulating factor (G-CSF) receptor (G-CSFR) is a type I cytokine receptor which is involved in hematopoietic cell maturation. G-CSFR has three putative C-mannosylation sites at W253, W318, and W446; however, it is not elucidated whether G-CSFR is C-mannosylated or not. In this study, we first demonstrated that G-CSFR was C-mannosylated at only W318. We also revealed that C-mannosylation of G-CSFR affects G-CSF-dependent downstream signaling through changing ligand binding capability but not cell surface localization. Moreover, C-mannosylation of G-CSFR was functional and regulated granulocytic differentiation in myeloid 32D cells. In conclusion, we found that G-CSFR is C-mannosylated at W318 and that this C-mannosylation has role(s) for myeloid cell differentiation through regulating downstream signaling.

Original languageEnglish
JournalBiochemical and Biophysical Research Communications
DOIs
Publication statusAccepted/In press - 2018 Jan 1

Keywords

  • C-mannosylation
  • Glycosylation
  • Granulocyte colony-stimulating factor receptor (G-CSFR)
  • Mass spectrometry
  • Myeloid differentiation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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