Regulation of inflammatory response of macrophages and induction of regulatory T cells by using retinoic acid-loaded nanostructured lipid carrier

Khadijah Zai, Narumi Ishihara, Hiroyuki Oguchi, Masato Hirota, Akihiro Kishimura, Takeshi Mori, Kouji Hase, Yoshiki Katayama

Research output: Contribution to journalArticle

Abstract

Immunomodulatory function of all-trans retinoic acid (ATRA) has been gathering much attention for the therapy of autoimmune diseases. ATRA is a chemically unstable molecule which requires proper formulation for targeted delivery. Here we examined nanostructured lipid carrier (NLC) for the formulation of ATRA. NLC is a representative nanoparticle formulation especially suited for oral delivery. We established the preparation procedures of ATRA-containing NLC (NLC-RA) which minimizes the degradation of ATRA during the preparation process. NLC-RA thus obtained was taken up by macrophages and induced anti-inflammatory response via suppressing NF-κB signaling as well as via enhancing the production of anti-inflammatory cytokines. Moreover, NLC-RA enhanced differentiation of naïve T cells to regulatory T cells in the co-culture system with dendritic cells. These results suggest that NLC-RA is a promising alternative therapy for the autoimmune diseases especially intestinal bowel disease.

Original languageEnglish
JournalJournal of Biomaterials Science, Polymer Edition
DOIs
Publication statusAccepted/In press - 2019 Jan 1

Fingerprint

T-cells
Macrophages
Regulatory T-Lymphocytes
Tretinoin
Lipids
Acids
Autoimmune Diseases
Anti-Inflammatory Agents
Intestinal Diseases
Complementary Therapies
Coculture Techniques
Cell culture
Nanoparticles
Dendritic Cells
Cytokines
T-Lymphocytes
Degradation
Molecules

Keywords

  • inflammatory response
  • macrophages
  • nanostructured lipid carrier
  • regulatory T cells
  • Retinoic acid

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Regulation of inflammatory response of macrophages and induction of regulatory T cells by using retinoic acid-loaded nanostructured lipid carrier. / Zai, Khadijah; Ishihara, Narumi; Oguchi, Hiroyuki; Hirota, Masato; Kishimura, Akihiro; Mori, Takeshi; Hase, Kouji; Katayama, Yoshiki.

In: Journal of Biomaterials Science, Polymer Edition, 01.01.2019.

Research output: Contribution to journalArticle

Zai, Khadijah ; Ishihara, Narumi ; Oguchi, Hiroyuki ; Hirota, Masato ; Kishimura, Akihiro ; Mori, Takeshi ; Hase, Kouji ; Katayama, Yoshiki. / Regulation of inflammatory response of macrophages and induction of regulatory T cells by using retinoic acid-loaded nanostructured lipid carrier. In: Journal of Biomaterials Science, Polymer Edition. 2019.
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