Abstract
Immunomodulatory function of all-trans retinoic acid (ATRA) has been gathering much attention for the therapy of autoimmune diseases. ATRA is a chemically unstable molecule which requires proper formulation for targeted delivery. Here we examined nanostructured lipid carrier (NLC) for the formulation of ATRA. NLC is a representative nanoparticle formulation especially suited for oral delivery. We established the preparation procedures of ATRA-containing NLC (NLC-RA) which minimizes the degradation of ATRA during the preparation process. NLC-RA thus obtained was taken up by macrophages and induced anti-inflammatory response via suppressing NF-κB signaling as well as via enhancing the production of anti-inflammatory cytokines. Moreover, NLC-RA enhanced differentiation of naïve T cells to regulatory T cells in the co-culture system with dendritic cells. These results suggest that NLC-RA is a promising alternative therapy for the autoimmune diseases especially intestinal bowel disease.
| Original language | English |
|---|---|
| Journal | Journal of Biomaterials Science, Polymer Edition |
| DOIs | |
| Publication status | Accepted/In press - 2019 Jan 1 |
Fingerprint
Keywords
- inflammatory response
- macrophages
- nanostructured lipid carrier
- regulatory T cells
- Retinoic acid
ASJC Scopus subject areas
- Biophysics
- Bioengineering
- Biomaterials
- Biomedical Engineering
Cite this
Regulation of inflammatory response of macrophages and induction of regulatory T cells by using retinoic acid-loaded nanostructured lipid carrier. / Zai, Khadijah; Ishihara, Narumi; Oguchi, Hiroyuki; Hirota, Masato; Kishimura, Akihiro; Mori, Takeshi; Hase, Kouji; Katayama, Yoshiki.
In: Journal of Biomaterials Science, Polymer Edition, 01.01.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Regulation of inflammatory response of macrophages and induction of regulatory T cells by using retinoic acid-loaded nanostructured lipid carrier
AU - Zai, Khadijah
AU - Ishihara, Narumi
AU - Oguchi, Hiroyuki
AU - Hirota, Masato
AU - Kishimura, Akihiro
AU - Mori, Takeshi
AU - Hase, Kouji
AU - Katayama, Yoshiki
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Immunomodulatory function of all-trans retinoic acid (ATRA) has been gathering much attention for the therapy of autoimmune diseases. ATRA is a chemically unstable molecule which requires proper formulation for targeted delivery. Here we examined nanostructured lipid carrier (NLC) for the formulation of ATRA. NLC is a representative nanoparticle formulation especially suited for oral delivery. We established the preparation procedures of ATRA-containing NLC (NLC-RA) which minimizes the degradation of ATRA during the preparation process. NLC-RA thus obtained was taken up by macrophages and induced anti-inflammatory response via suppressing NF-κB signaling as well as via enhancing the production of anti-inflammatory cytokines. Moreover, NLC-RA enhanced differentiation of naïve T cells to regulatory T cells in the co-culture system with dendritic cells. These results suggest that NLC-RA is a promising alternative therapy for the autoimmune diseases especially intestinal bowel disease.
AB - Immunomodulatory function of all-trans retinoic acid (ATRA) has been gathering much attention for the therapy of autoimmune diseases. ATRA is a chemically unstable molecule which requires proper formulation for targeted delivery. Here we examined nanostructured lipid carrier (NLC) for the formulation of ATRA. NLC is a representative nanoparticle formulation especially suited for oral delivery. We established the preparation procedures of ATRA-containing NLC (NLC-RA) which minimizes the degradation of ATRA during the preparation process. NLC-RA thus obtained was taken up by macrophages and induced anti-inflammatory response via suppressing NF-κB signaling as well as via enhancing the production of anti-inflammatory cytokines. Moreover, NLC-RA enhanced differentiation of naïve T cells to regulatory T cells in the co-culture system with dendritic cells. These results suggest that NLC-RA is a promising alternative therapy for the autoimmune diseases especially intestinal bowel disease.
KW - inflammatory response
KW - macrophages
KW - nanostructured lipid carrier
KW - regulatory T cells
KW - Retinoic acid
UR - http://www.scopus.com/inward/record.url?scp=85060219324&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85060219324&partnerID=8YFLogxK
U2 - 10.1080/09205063.2018.1493671
DO - 10.1080/09205063.2018.1493671
M3 - Article
C2 - 29943678
AN - SCOPUS:85060219324
JO - Journal of Biomaterials Science, Polymer Edition
JF - Journal of Biomaterials Science, Polymer Edition
SN - 0920-5063
ER -