Regulation of mouse retinol dehydrogenases and retinal dehydrogenases in hepatocyte differentiation.

Kengo Tomita, Toshifumi Azuma, Sayaka Inokuchi, Naoto Kitamura, Takeshi Nishimura, Shinzo Kato, Hiromasa Ishii

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Retinoic acid (RA) is produced via two sequential reactions of retinol dehydrogenases (RDHs) and retinal dehydrogenases (RALDHs). We found that primary cultured mouse hepatocytes on a single collagen gel gradually lost hepatocyte specific morphology, and that another collagen gel overlay remarkably recovered it. The levels of albumin and liver-dominant expressed RDHs expression in hepatocytes paralleled their morphological change, decreased during single collagen gel culture, and were up-regulated by sequential collagen overlay. Quite similar to the expression changes, albumin and those RDHs' mRNA expression levels increased along with liver differentiation during pre- and postnatal liver development. Our data supports that all-trans and 9-cis RA, catalyzed by the RDHs, indeed play an important role in liver differentiation and regeneration.

Original languageEnglish
Pages (from-to)46-57
Number of pages12
JournalNihon Arukoru Yakubutsu Igakkai zasshi = Japanese journal of alcohol studies & drug dependence
Volume38
Issue number1
Publication statusPublished - 2003 Feb

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Retinal Dehydrogenase
Hepatocytes
Collagen
regulation
Gels
Albumins
Liver
Liver Regeneration
Tretinoin
Messenger RNA
retinol dehydrogenase

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Health(social science)

Cite this

Regulation of mouse retinol dehydrogenases and retinal dehydrogenases in hepatocyte differentiation. / Tomita, Kengo; Azuma, Toshifumi; Inokuchi, Sayaka; Kitamura, Naoto; Nishimura, Takeshi; Kato, Shinzo; Ishii, Hiromasa.

In: Nihon Arukoru Yakubutsu Igakkai zasshi = Japanese journal of alcohol studies & drug dependence, Vol. 38, No. 1, 02.2003, p. 46-57.

Research output: Contribution to journalArticle

Tomita, Kengo ; Azuma, Toshifumi ; Inokuchi, Sayaka ; Kitamura, Naoto ; Nishimura, Takeshi ; Kato, Shinzo ; Ishii, Hiromasa. / Regulation of mouse retinol dehydrogenases and retinal dehydrogenases in hepatocyte differentiation. In: Nihon Arukoru Yakubutsu Igakkai zasshi = Japanese journal of alcohol studies & drug dependence. 2003 ; Vol. 38, No. 1. pp. 46-57.
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