Regulation of notochord-specific expression of Ci-Bra downstream genes in Ciona intestinalis embryos

Hiroki Takahashi, Kohji Hotta, Chiyo Takagi, Naoto Ueno, Nori Satoh, Eiichi Shoguchi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Brachyury, a T-box transcription factor, is expressed in ascidian embryos exclusively in primordial notochord cells and plays a pivotal role in differentiation of notochord cells. Previously, we identified ∼450 genes downstream of Ciona intestinalis Brachyury (Ci-Bra), and characterized the expression profiles of 45 of these in differentiating notochord cells. In this study, we looked for cisregulatory sequences in minimal enhancers of 20 Ci-Bra downstream genes by electroporating region within ∼3 kb upstream of each gene fused with lacZ. Eight of the 20 reporters were expressed in notochord cells. The minimal enchancer for each of these eight genes was narrowed to a region ∼0.51.0-kb long. We also explored the genome-wide and coordinate regulation of 43 Ci-Bra-downstream genes. When we determined their chromosomal localization, it became evident that they are not clustered in a given region of the genome, but rather distributed evenly over 13 of the 14 pairs of chromosomes, suggesting that gene clustering does not contribute to coordinate control of the Ci-Bra downstream gene expression. Our results might provide Insights Into the molecular mechanisms underlying notochord formation in chordates.

Original languageEnglish
Pages (from-to)110-118
Number of pages9
JournalZoological Science
Volume27
Issue number2
DOIs
Publication statusPublished - 2010 Feb

Fingerprint

embryo (animal)
genes
enhancer elements
Chordata
genome
Ascidiacea
cells
cell differentiation
Ciona intestinalis
transcription factors
chromosomes
gene expression

Keywords

  • Ci-Bra
  • Ciona intestinalis
  • Cis-regulatory regions
  • Downstream genes
  • Genome-wide regulation
  • Notochord differentiation

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Regulation of notochord-specific expression of Ci-Bra downstream genes in Ciona intestinalis embryos. / Takahashi, Hiroki; Hotta, Kohji; Takagi, Chiyo; Ueno, Naoto; Satoh, Nori; Shoguchi, Eiichi.

In: Zoological Science, Vol. 27, No. 2, 02.2010, p. 110-118.

Research output: Contribution to journalArticle

Takahashi, Hiroki ; Hotta, Kohji ; Takagi, Chiyo ; Ueno, Naoto ; Satoh, Nori ; Shoguchi, Eiichi. / Regulation of notochord-specific expression of Ci-Bra downstream genes in Ciona intestinalis embryos. In: Zoological Science. 2010 ; Vol. 27, No. 2. pp. 110-118.
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