Regulation of the HIF-1α level is essential for hematopoietic stem cells

Keiyo Takubo, Nobuhito Goda, Wakako Yamasawa, Hirono Iriuchishima, Eiji Ikeda, Yoshiaki Kubota, Haruko Shima, Randall S. Johnson, Atsushi Hirao, Makoto Suematsu, Toshio Suda

Research output: Contribution to journalArticle

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Abstract

Hematopoietic stem cells (HSCs) are sustained in a specific microenvironment known as the stem cell niche. Mammalian HSCs are kept quiescent in the endosteal niche, a hypoxic zone of the bone marrow (BM). In this study, we show that normal HSCs maintain intracellular hypoxia and stabilize hypoxia-inducible factor-1α (HIF-1α) protein. In HIF-1α-deficient mice, the HSCs lost their cell cycle quiescence and HSC numbers decreased during various stress settings including bone marrow transplantation, myelosuppression, or aging, in a p16Ink4a/p19 Arf-dependent manner. Overstabilization of HIF-1α by biallelic loss of an E3 ubiquitin ligase for HIF-1α (VHL) induced cell cycle quiescence in HSCs and their progenitors but resulted in an impairment in transplantation capacity. In contrast, monoallelic loss of VHL induced cell cycle quiescence and improved BM engraftment during bone marrow transplantation. These data indicate that HSCs maintain cell cycle quiescence through the precise regulation of HIF-1α levels.

Original languageEnglish
Pages (from-to)391-402
Number of pages12
JournalCell Stem Cell
Volume7
Issue number3
DOIs
Publication statusPublished - 2010 Sep 3

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Hypoxia-Inducible Factor 1
Hematopoietic Stem Cells
Cell Cycle
Bone Marrow Transplantation
Bone Marrow
Stem Cell Niche
Ubiquitin-Protein Ligases
Cell Count
Transplantation

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Medicine
  • Genetics

Cite this

Regulation of the HIF-1α level is essential for hematopoietic stem cells. / Takubo, Keiyo; Goda, Nobuhito; Yamasawa, Wakako; Iriuchishima, Hirono; Ikeda, Eiji; Kubota, Yoshiaki; Shima, Haruko; Johnson, Randall S.; Hirao, Atsushi; Suematsu, Makoto; Suda, Toshio.

In: Cell Stem Cell, Vol. 7, No. 3, 03.09.2010, p. 391-402.

Research output: Contribution to journalArticle

Takubo, K, Goda, N, Yamasawa, W, Iriuchishima, H, Ikeda, E, Kubota, Y, Shima, H, Johnson, RS, Hirao, A, Suematsu, M & Suda, T 2010, 'Regulation of the HIF-1α level is essential for hematopoietic stem cells', Cell Stem Cell, vol. 7, no. 3, pp. 391-402. https://doi.org/10.1016/j.stem.2010.06.020
Takubo, Keiyo ; Goda, Nobuhito ; Yamasawa, Wakako ; Iriuchishima, Hirono ; Ikeda, Eiji ; Kubota, Yoshiaki ; Shima, Haruko ; Johnson, Randall S. ; Hirao, Atsushi ; Suematsu, Makoto ; Suda, Toshio. / Regulation of the HIF-1α level is essential for hematopoietic stem cells. In: Cell Stem Cell. 2010 ; Vol. 7, No. 3. pp. 391-402.
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