Regulatory effect of interleukin-4 and interleukin-13 on colon cancer cell adhesion

Takanori Kanai, M. Watanabe, A. Hayashi, A. Nakazawa, T. Yajima, A. Okazawa, M. Yamazaki, H. Ishii, T. Hibi

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

To assess the role of interleukin-4 (IL-4) and interleukin-13 (IL-13) in colon cancer cell-cell adhesion, we investigated the effect of both cytokines in human colon cancer cell line, colo205 cell-cell adhesion. IL-4 receptor was expressed on the cell surface of colo205, and recombinant IL-4 inhibited colo205 cell-cell adhesion in a dose-dependent fashion without inhibiting cell proliferation. Flow cytometric analysis revealed that monoclonal antibodies (mAbs) directed against E-cadherin and carcinoembryonic antigen (CEA) inhibited colo205 cell-cell adhesion and IL-4 significantly inhibited the expression of E-cadherin and CEA. IL-13 also inhibited colo205 cell-cell adhesion. These results indicated that IL-4 and IL-13 inhibited colon cancer cell-cell adhesion by down-regulation of E-cadherin and CEA molecules. We then investigated the expression of both cytokines from freshly isolated colon cancer tumour-infiltrating lymphocytes (TILs). With reverse transcription-polymerase chain reaction and flow cytometric analysis. we demonstrated that colon TILs expressed IL-4 and IL-13 mRNA and protein. These results suggest that Th 2 type cytokines IL-4 and IL-13 locally-produced from TILs may regulate colon cancer adhesion by down-regulation of adhesion molecules. (C) 2000 Cancer Research Campaign.

Original languageEnglish
Pages (from-to)1717-1723
Number of pages7
JournalBritish Journal of Cancer
Volume82
Issue number10
Publication statusPublished - 2000

Fingerprint

Interleukin-13
Cell Adhesion
Interleukin-4
Colonic Neoplasms
Tumor-Infiltrating Lymphocytes
Carcinoembryonic Antigen
Cadherins
Cytokines
Down-Regulation
Interleukin-4 Receptors
Reverse Transcription
Colon
Monoclonal Antibodies
Cell Proliferation
Cell Line
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Cell adhesion
  • Colon cancer
  • IL-13
  • IL-4

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Kanai, T., Watanabe, M., Hayashi, A., Nakazawa, A., Yajima, T., Okazawa, A., ... Hibi, T. (2000). Regulatory effect of interleukin-4 and interleukin-13 on colon cancer cell adhesion. British Journal of Cancer, 82(10), 1717-1723.

Regulatory effect of interleukin-4 and interleukin-13 on colon cancer cell adhesion. / Kanai, Takanori; Watanabe, M.; Hayashi, A.; Nakazawa, A.; Yajima, T.; Okazawa, A.; Yamazaki, M.; Ishii, H.; Hibi, T.

In: British Journal of Cancer, Vol. 82, No. 10, 2000, p. 1717-1723.

Research output: Contribution to journalArticle

Kanai, T, Watanabe, M, Hayashi, A, Nakazawa, A, Yajima, T, Okazawa, A, Yamazaki, M, Ishii, H & Hibi, T 2000, 'Regulatory effect of interleukin-4 and interleukin-13 on colon cancer cell adhesion', British Journal of Cancer, vol. 82, no. 10, pp. 1717-1723.
Kanai T, Watanabe M, Hayashi A, Nakazawa A, Yajima T, Okazawa A et al. Regulatory effect of interleukin-4 and interleukin-13 on colon cancer cell adhesion. British Journal of Cancer. 2000;82(10):1717-1723.
Kanai, Takanori ; Watanabe, M. ; Hayashi, A. ; Nakazawa, A. ; Yajima, T. ; Okazawa, A. ; Yamazaki, M. ; Ishii, H. ; Hibi, T. / Regulatory effect of interleukin-4 and interleukin-13 on colon cancer cell adhesion. In: British Journal of Cancer. 2000 ; Vol. 82, No. 10. pp. 1717-1723.
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