Regulatory effect of interleukin-4 and interleukin-13 on colon cancer cell adhesion

T. Kanai, M. Watanabe, A. Hayashi, A. Nakazawa, T. Yajima, A. Okazawa, M. Yamazaki, H. Ishii, T. Hibi

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50 Citations (Scopus)

Abstract

To assess the role of interleukin-4 (IL-4) and interleukin-13 (IL-13) in colon cancer cell-cell adhesion, we investigated the effect of both cytokines in human colon cancer cell line, colo205 cell-cell adhesion. IL-4 receptor was expressed on the cell surface of colo205, and recombinant IL-4 inhibited colo205 cell-cell adhesion in a dose-dependent fashion without inhibiting cell proliferation. Flow cytometric analysis revealed that monoclonal antibodies (mAbs) directed against E-cadherin and carcinoembryonic antigen (CEA) inhibited colo205 cell-cell adhesion and IL-4 significantly inhibited the expression of E-cadherin and CEA. IL-13 also inhibited colo205 cell-cell adhesion. These results indicated that IL-4 and IL-13 inhibited colon cancer cell-cell adhesion by down-regulation of E-cadherin and CEA molecules. We then investigated the expression of both cytokines from freshly isolated colon cancer tumour-infiltrating lymphocytes (TILs). With reverse transcription-polymerase chain reaction and flow cytometric analysis. we demonstrated that colon TILs expressed IL-4 and IL-13 mRNA and protein. These results suggest that Th 2 type cytokines IL-4 and IL-13 locally-produced from TILs may regulate colon cancer adhesion by down-regulation of adhesion molecules. (C) 2000 Cancer Research Campaign.

Original languageEnglish
Pages (from-to)1717-1723
Number of pages7
JournalBritish Journal of Cancer
Volume82
Issue number10
Publication statusPublished - 2000

Keywords

  • Cell adhesion
  • Colon cancer
  • IL-13
  • IL-4

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Kanai, T., Watanabe, M., Hayashi, A., Nakazawa, A., Yajima, T., Okazawa, A., Yamazaki, M., Ishii, H., & Hibi, T. (2000). Regulatory effect of interleukin-4 and interleukin-13 on colon cancer cell adhesion. British Journal of Cancer, 82(10), 1717-1723.