Regulatory mechanism of granulopoiesis in the bone marrow of CSF-producing tumor-bearing nude mice

K. Motoyoshi, T. Suda, F. Takaku, Y. Miura

Research output: Contribution to journalArticle

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Abstract

The regulatory mechanism of differentiation of granulocyte and macrophage precursor cells (G/M CFU-C) in bone marrow and spleen obtained from nude mice bearing colony stimulating factor (CSF) producing tumor (G-mice). which developed marked granulocytosis, was studied. In these mice granulopoiesis is enhanced in the spleen, but suppressed in bone marrow. Coculture of G-mouse bone marrow cells with splenic cells of control nude mice (C-mice) and of G-mice resulted in 68% and 62%, respectively, more colonies than expected, while coculture of C-mouse bone marrow cells with these two sources of cell fractions resulted in only 2% and 11% more colonies. In the double-layer agar culture system, bone marrow and splenic cells of C- and G-mice produced a maximal number of colonies containing adequate amounts of human urinary CSF in the upper layer when C-mouse bone marrow cells were added to the lower layer, while these four sources of cells produced a moderate or minimal number of colonies when splenic cells of C- and G-mice or G-mouse bone marrow cells were added to the lower layer. Morphological examination of colonies formed in the upper layer revealed that addition of C-mouse bone marrow cells or irradiated G-mouse bone marrow cells to the lower layer resulted in a massive increment in the number of colonies with pure granulocytes and granulocyte and macrophage mixed (G + G/M) colonies formed by G-mouse bone marrow cells in the upper layer. However, the addition of irradiated C-mouse bone marrow cells or G-mouse bone marrow cells before irradiation to the lower layer did not change G + G/M colony formation by G-mouse bone marrow cells in the upper layer. We could reproduce these findings with conditioned media obtained from 3-day liquid cultures of these cell fractions. This suggests that a diffusible factor may be necessary for inhibition of G + G/M colony formation in G-mouse bone marrow cells. The fine mechanism of such inhibition remains to be clarified.

Original languageEnglish
Pages (from-to)980-987
Number of pages8
JournalBlood
Volume62
Issue number5
Publication statusPublished - 1983

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Bearings (structural)
Colony-Stimulating Factors
Nude Mice
Tumors
Bone
Bone Marrow
Bone Marrow Cells
Cells
Neoplasms
Macrophages
Coculture Techniques
Granulocytes
Spleen

ASJC Scopus subject areas

  • Hematology

Cite this

Motoyoshi, K., Suda, T., Takaku, F., & Miura, Y. (1983). Regulatory mechanism of granulopoiesis in the bone marrow of CSF-producing tumor-bearing nude mice. Blood, 62(5), 980-987.

Regulatory mechanism of granulopoiesis in the bone marrow of CSF-producing tumor-bearing nude mice. / Motoyoshi, K.; Suda, T.; Takaku, F.; Miura, Y.

In: Blood, Vol. 62, No. 5, 1983, p. 980-987.

Research output: Contribution to journalArticle

Motoyoshi, K, Suda, T, Takaku, F & Miura, Y 1983, 'Regulatory mechanism of granulopoiesis in the bone marrow of CSF-producing tumor-bearing nude mice', Blood, vol. 62, no. 5, pp. 980-987.
Motoyoshi, K. ; Suda, T. ; Takaku, F. ; Miura, Y. / Regulatory mechanism of granulopoiesis in the bone marrow of CSF-producing tumor-bearing nude mice. In: Blood. 1983 ; Vol. 62, No. 5. pp. 980-987.
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