Rejoining of isochromatid breaks induced by heavy ions in G2-phase normal human fibroblasts

T. Kawata, M. Durante, Y. Furusawa, K. George, H. Ito, H. Wu, F. A. Cucinotta

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Abstract

We reported previously that exposure of normal human fibroblasts in G2 phase of the cell cycle to high-LET radiation produces a much higher frequency of isochromatid breaks than exposure to γ rays. We concluded that an increase in the production of isochromatid breaks is a signature of initial high-LET radiation-induced G2-phase damage. In this paper, we report the repair kinetics of isochromatid breaks induced by high-LET radiation in normal G2-phase human fibroblasts. Exponentially growing human fibroblasts (AG1522) were irradiated with γ rays or energetic carbon (290 MeV/nucleon), silicon (490 MeV/nucleon), or iron (200 MeV/nucleon) ions. Prematurely condensed chromosomes were induced by calyculin A after different postirradiation incubation times ranging from 0 to 600 min. Chromosomes were stained with Giemsa, and aberrations were scored in cells at G2 phase. G2-phase fragments, the result of the induction of isochromatid breaks, decreased quickly with incubation time. The curve for the kinetics of the rejoining of chromatid-type breaks showed a slight upward curvature with time after exposure to 440 keV/μm iron particles, probably due to isochromatid-isochromatid break rejoining. The formation of chromatid exchanges after exposure to high-LET radiation therefore appears to be underestimated, because isochromatid-isochromatid exchanges cannot be detected. Increased induction of isochromatid breaks and rejoining of isochromatid breaks affect the overall kinetics of chromatid-type break rejoining after exposure to high-LET radiation.

Original languageEnglish
Pages (from-to)598-602
Number of pages5
JournalRadiation Research
Volume156
Issue number5 II
DOIs
Publication statusPublished - 2001

ASJC Scopus subject areas

  • Biophysics
  • Radiation
  • Radiology Nuclear Medicine and imaging

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    Kawata, T., Durante, M., Furusawa, Y., George, K., Ito, H., Wu, H., & Cucinotta, F. A. (2001). Rejoining of isochromatid breaks induced by heavy ions in G2-phase normal human fibroblasts. Radiation Research, 156(5 II), 598-602. https://doi.org/10.1667/0033-7587(2001)156[0598:roibib]2.0.co;2