Relationship between neutrophil influx and oxidative stress in alveolar space in lipopolysaccharide-induced lung injury

T. Yoshida, K. Nagai, T. Inomata, Y. Ito, Tomoko Betsuyaku, M. Nishimura

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We intratracheally administered lipopolysaccharide (LPS) to ICR mice and then collected BAL fluid and lung tissue to determine whether levels of neutrophils and/or myeloperoxidase (MPO) in bronchoalveolar lavage (BAL) fluid reflect lung tissue damage. Robust neutrophil accumulation into the alveolar space and lung tissue were almost completely abolished at seven days along with oxidative stress markers in the lung. However, lung injury scores and lung wet/dry ratios, as well as MPO and oxidative stress markers in BAL fluid were significantly increased at five and seven days after LPS administration. At later time points, BAL neutrophils generated more MPO activity and ROS than those harvested sooner after LPS administration. Although elevated neutrophil levels in BAL fluid reflected oxidative stress in the lungs, MPO might serve as a useful marker to evaluate damage sustained by epithelial cells over the long term.

Original languageEnglish
Pages (from-to)75-83
Number of pages9
JournalRespiratory Physiology and Neurobiology
Volume191
Issue number1
DOIs
Publication statusPublished - 2014 Jan 15

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Keywords

  • Lung injury model
  • Myeloperoxidase
  • Neutrophil recruitment
  • Oxidative stress marker
  • Reactive oxygen species

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Neuroscience(all)
  • Medicine(all)

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