Relationship of aberrant DNA hypermethylation of CHFR with sensitivity to taxanes in endometrial cancer

Megumi Yanokura, Kouji Banno, Makiko Kawaguchi, Nobumaru Hirao, Akira Hirasawa, Nobuyuki Susumu, Katsumi Tsukazaki, Daisuke Aoki

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The relationship of aberrant DNA hypermethylation of cell cycle checkpoint genes with the sensitivity of cancer cells to anticancer drugs is a question of current interest. In this study, we investigated the relationship between aberrant hypermethylation of the CHFR (checkpoint with forkhead-associated and ring finger) mitotic checkpoint gene and sensitivity to taxanes in endometrial cancer. Methylation-specific PCR (MSP) indicated aberrant hypermethylation of CHFR in 12.0% (6/50) of endometrial cancer specimens, and suggested that aberrant hypermethylation is significantly more frequent in poorly differentiated adenocarcinoma (G3) (p<0.05). Of six culture cell lines, SNG-II and HEC108 cells showed aberrant hypermethylation and reduced expression of CHFR. These cells had high sensitivity to taxanes but became resistant after demethylation. Cancer specimens with aberrant hypermethylation of CHFR also exhibited high sensitivity to taxanes. To our knowledge, this study is the first to examine aberrant hypermethylation of CHFR in endometrial cancer, and our results suggest that the methylation status of CHFR may be a new molecular index that will allow design of personalized treatment in endometrial cancer. This may be particularly important in poorly differentiated adenocarcinoma (G3), which is known to have a poor prognosis.

Original languageEnglish
Pages (from-to)41-48
Number of pages8
JournalOncology Reports
Volume17
Issue number1
Publication statusPublished - 2007 Jan

Fingerprint

Taxoids
Endometrial Neoplasms
DNA
Methylation
Adenocarcinoma
M Phase Cell Cycle Checkpoints
cdc Genes
Fingers
Neoplasms
Cell Line
Polymerase Chain Reaction
Pharmaceutical Preparations
Genes

Keywords

  • Chemosensitivity
  • CHFR
  • DNA hypermethylation
  • Endometrial cancer
  • Taxane

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Relationship of aberrant DNA hypermethylation of CHFR with sensitivity to taxanes in endometrial cancer. / Yanokura, Megumi; Banno, Kouji; Kawaguchi, Makiko; Hirao, Nobumaru; Hirasawa, Akira; Susumu, Nobuyuki; Tsukazaki, Katsumi; Aoki, Daisuke.

In: Oncology Reports, Vol. 17, No. 1, 01.2007, p. 41-48.

Research output: Contribution to journalArticle

Yanokura, M, Banno, K, Kawaguchi, M, Hirao, N, Hirasawa, A, Susumu, N, Tsukazaki, K & Aoki, D 2007, 'Relationship of aberrant DNA hypermethylation of CHFR with sensitivity to taxanes in endometrial cancer', Oncology Reports, vol. 17, no. 1, pp. 41-48.
Yanokura M, Banno K, Kawaguchi M, Hirao N, Hirasawa A, Susumu N et al. Relationship of aberrant DNA hypermethylation of CHFR with sensitivity to taxanes in endometrial cancer. Oncology Reports. 2007 Jan;17(1):41-48.
Yanokura, Megumi ; Banno, Kouji ; Kawaguchi, Makiko ; Hirao, Nobumaru ; Hirasawa, Akira ; Susumu, Nobuyuki ; Tsukazaki, Katsumi ; Aoki, Daisuke. / Relationship of aberrant DNA hypermethylation of CHFR with sensitivity to taxanes in endometrial cancer. In: Oncology Reports. 2007 ; Vol. 17, No. 1. pp. 41-48.
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