Reliability of Small Biopsy Samples Compared With Resected Specimens for the Determination of Programmed Death-Ligand 1 Expression in Non-Small-Cell Lung Cancer

Satoru Kitazono, Yutaka Fujiwara, Koji Tsuta, Hirofumi Utsumi, Shintaro Kanda, Hidehito Horinouchi, Hiroshi Nokihara, Noboru Yamamoto, Shinji Sasada, Shun Ichi Watanabe, Hisao Asamura, Tomohide Tamura, Yuichiro Ohe

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Abstract

Background Several studies have assessed the expression of programmed death-ligand 1 (PD-L1) in resected surgical specimens of non-small-cell lung cancer (NSCLC). However, the expression of PD-L1 in smaller biopsy samples of advanced NSCLC has not been reported. Patients and Methods A total of 79 patients with NSCLC at our institution with available biopsy samples and resected specimens were retrospectively enrolled in the present study. PD-L1 expression was assessed by immunohistochemistry and scored using the hybrid scoring method. The concordance rates for the expression of PD-L1 between the 2 samples were analyzed. Results The pathologic stage of the patients (51 men, 28 women; median age, 68 years) was stage I in 37, stage II in 18, and stage III in 24. The diagnostic procedures included transbronchial biopsy in 59, transbronchial needle aspiration biopsy in 14, and computed tomography (CT)-guided needle biopsy in 6. The positivity rate of PD-L1 in these samples was 38.0% (27 transbronchial biopsies, 6 transbronchial needle aspiration biopsies, 3 CT-guided needle biopsies) versus 35.4% in the resected specimens. The median hybrid score was 0 (range, 0-170), and the mean score was 28.7 ± 43.4. Comparing the biopsy samples and resected specimens with a score of ≥ 1 as positive for PD-L1 staining, 6 tumors were discordant for PD-L1 expression and 73 were concordant, for a concordance rate of 92.4% and κ value of 0.8366. Conclusion PD-L1 status showed good concordance between the biopsy samples and resected specimens. These small samples, even those derived from transbronchial needle aspiration biopsies, appear adequate for the assessment of PD-L1 expression.

Original languageEnglish
Article number369
Pages (from-to)385-390
Number of pages6
JournalClinical Lung Cancer
Volume16
Issue number5
DOIs
Publication statusPublished - 2015 Sep 1
Externally publishedYes

Keywords

  • Concordance rate
  • Immunohistochemistry
  • Operative
  • Surgical procedures

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pulmonary and Respiratory Medicine

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    Kitazono, S., Fujiwara, Y., Tsuta, K., Utsumi, H., Kanda, S., Horinouchi, H., Nokihara, H., Yamamoto, N., Sasada, S., Watanabe, S. I., Asamura, H., Tamura, T., & Ohe, Y. (2015). Reliability of Small Biopsy Samples Compared With Resected Specimens for the Determination of Programmed Death-Ligand 1 Expression in Non-Small-Cell Lung Cancer. Clinical Lung Cancer, 16(5), 385-390. [369]. https://doi.org/10.1016/j.cllc.2015.03.008