RELIABLE RE-FORMATION OF A LIPID BILAYER USING A GEOMETRICALLY GUIDED AIR BUBBLE

Izumi Hashimoto, Toshihisa Osaki, Hirotaka Sugiura, Hisatoshi Mimura, Norihisa Miki, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports successful re-formation of a lipid bilayer using an air bubble. Lipid bilayers have been used for membrane protein studies and biosensor applications. Droplet-contact-method is a simple and effective method to form the bilayers. A major challenge in using the bilayers originates from their vulnerability against physical and electrical disturbance, which leads the bilayer rapture and the droplet fusion. We previously proposed a concept to re-form a bilayer by introducing an air bubble to cut-and-split the merged droplet. In this work, we investigated the key parameters to guide the bubble and drastically enhanced the yield up to 97%.

Original languageEnglish
Title of host publicationMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages351-352
Number of pages2
ISBN (Electronic)9781733419031
Publication statusPublished - 2021
Event25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States
Duration: 2021 Oct 102021 Oct 14

Publication series

NameMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Country/TerritoryUnited States
CityPalm Springs, Virtual
Period21/10/1021/10/14

Keywords

  • Air bubble
  • Lipid bilayer membrane
  • Re-formation
  • Robustness

ASJC Scopus subject areas

  • Bioengineering
  • Chemical Engineering (miscellaneous)

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