Remarkable activities of nucleoside triphosphate hydrolase in the tachyzoites of both virulent and avirulent strains of Toxoplasma gondii

Takashi Asai; Yasuhiro Suzuki

doi:10.1016/0378-1097(90)90350-Y

Remarkable activities of nucleoside triphosphate hydrolase in the tachyzoites of both virulent and avirulent strains of Toxoplasma gondii

Takashi Asai, Yasuhiro Suzuki

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Dithiothreitol-dependent MgATP² and MgADP^- hydrolytic activities owing to nucleoside triphosphate hydrolase were examined kinetically un tachyzoite cells of five strains of Toxoplasma gondii with various virulence. The tachyzoites of all the strains were revealed to have a high ATP and ADP hydrolytic potency. The value of V_max and K_m obtained from each strain indicated that there were two classified types of T. gondii about ATP and ADP hydrolysis. The most virulent strain (RH) and avirulent strain (Fukaya) were classified in a type with higher ATPase than ADPase activities. Two virulent strains (C56, Beverley) and an avirulent strain (ME49) were classified in the other type with the same activities of ATPase and ADPase.

Original language	English
Pages (from-to)	89-92
Number of pages	4
Journal	FEMS microbiology letters
Volume	72
Issue number	1-2
DOIs	https://doi.org/10.1016/0378-1097(90)90350-Y
Publication status	Published - 1990 Oct
Externally published	Yes

Keywords

ADPase
ATPase
Nucleoside triphosphate hydrolase
Toxoplasma gondii

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

Access to Document

10.1016/0378-1097(90)90350-Y

Cite this

@article{37bb8c843733439f93e78f04901803cc,

title = "Remarkable activities of nucleoside triphosphate hydrolase in the tachyzoites of both virulent and avirulent strains of Toxoplasma gondii",

abstract = "Dithiothreitol-dependent MgATP2 and MgADP- hydrolytic activities owing to nucleoside triphosphate hydrolase were examined kinetically un tachyzoite cells of five strains of Toxoplasma gondii with various virulence. The tachyzoites of all the strains were revealed to have a high ATP and ADP hydrolytic potency. The value of Vmax and Km obtained from each strain indicated that there were two classified types of T. gondii about ATP and ADP hydrolysis. The most virulent strain (RH) and avirulent strain (Fukaya) were classified in a type with higher ATPase than ADPase activities. Two virulent strains (C56, Beverley) and an avirulent strain (ME49) were classified in the other type with the same activities of ATPase and ADPase.",

keywords = "ADPase, ATPase, Nucleoside triphosphate hydrolase, Toxoplasma gondii",

author = "Takashi Asai and Yasuhiro Suzuki",

note = "Funding Information: Tb~s wor~, wa~ supported in part by Grant-in-Aid for Encouragement of Young Scientists 01770266 from the Ministry of Education, Science a,'~d Culture of Japan, and also supported in part by a grant from Ohm,a rea Health Foundation. We thank Dr. M. Kobayashi (Chiba University) 1or providing us a computer program for K,, and V,,,~ determination.",

year = "1990",

month = oct,

doi = "10.1016/0378-1097(90)90350-Y",

language = "English",

volume = "72",

pages = "89--92",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Wiley-Blackwell",

number = "1-2",

}

TY - JOUR

T1 - Remarkable activities of nucleoside triphosphate hydrolase in the tachyzoites of both virulent and avirulent strains of Toxoplasma gondii

AU - Asai, Takashi

AU - Suzuki, Yasuhiro

N1 - Funding Information: Tb~s wor~, wa~ supported in part by Grant-in-Aid for Encouragement of Young Scientists 01770266 from the Ministry of Education, Science a,'~d Culture of Japan, and also supported in part by a grant from Ohm,a rea Health Foundation. We thank Dr. M. Kobayashi (Chiba University) 1or providing us a computer program for K,, and V,,,~ determination.

PY - 1990/10

Y1 - 1990/10

N2 - Dithiothreitol-dependent MgATP2 and MgADP- hydrolytic activities owing to nucleoside triphosphate hydrolase were examined kinetically un tachyzoite cells of five strains of Toxoplasma gondii with various virulence. The tachyzoites of all the strains were revealed to have a high ATP and ADP hydrolytic potency. The value of Vmax and Km obtained from each strain indicated that there were two classified types of T. gondii about ATP and ADP hydrolysis. The most virulent strain (RH) and avirulent strain (Fukaya) were classified in a type with higher ATPase than ADPase activities. Two virulent strains (C56, Beverley) and an avirulent strain (ME49) were classified in the other type with the same activities of ATPase and ADPase.

AB - Dithiothreitol-dependent MgATP2 and MgADP- hydrolytic activities owing to nucleoside triphosphate hydrolase were examined kinetically un tachyzoite cells of five strains of Toxoplasma gondii with various virulence. The tachyzoites of all the strains were revealed to have a high ATP and ADP hydrolytic potency. The value of Vmax and Km obtained from each strain indicated that there were two classified types of T. gondii about ATP and ADP hydrolysis. The most virulent strain (RH) and avirulent strain (Fukaya) were classified in a type with higher ATPase than ADPase activities. Two virulent strains (C56, Beverley) and an avirulent strain (ME49) were classified in the other type with the same activities of ATPase and ADPase.

KW - ADPase

KW - ATPase

KW - Nucleoside triphosphate hydrolase

KW - Toxoplasma gondii

UR - http://www.scopus.com/inward/record.url?scp=0025134672&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025134672&partnerID=8YFLogxK

U2 - 10.1016/0378-1097(90)90350-Y

DO - 10.1016/0378-1097(90)90350-Y

M3 - Article

C2 - 2149344

AN - SCOPUS:0025134672

SN - 0378-1097

VL - 72

SP - 89

EP - 92

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1-2

ER -

Remarkable activities of nucleoside triphosphate hydrolase in the tachyzoites of both virulent and avirulent strains of Toxoplasma gondii

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this