Remarkable genetic polymorphism among Entamoeba histolytica isolates from a limited geographic area

Ali Haghighi, Seiki Kobayashi, Tsutomu Takeuchi, Gohta Masuda, Tomoyoshi Nozaki

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

In order to understand genetic polymorphisms among Entamoeba histolytica strains in a limited geographic area and among restricted social populations, we studied nucleotide polymorphism in DNA regions that do not encode proteins (locus 1-2 and locus 5-6) and in genes coding for chitinase and for serine-rich E. histolytica protein. Thirty E. histolytica isolates from domestically infected Japanese amebiasis patients (male homosexuals and residents in institutions for the mentally handicapped) and four reference strains were examined. PCR revealed remarkable polymorphisms in both the number and size of the PCR fragments containing these loci. Polymorphisms in lengths, types, and numbers of internal repeat units were observed in locus 1-2 and the repeat-containing region of serine-rich E. histolytica protein among the Japanese isolates. In contrast, polymorphism at locus 5-6 was observed almost exclusively in the number of repeats of a 16-nucleotide unit. The repeat-containing region of chitinase appeared to be the least polymorphic among the four loci with a single dominant genotype representing 66% (20 out of 30) of all of the isolates. Isolates obtained from male homosexuals showed a more complex genetic polymorphism than those from residents in institutions. Considering all four polymorphic loci together, all 19 Japanese isolates from male homosexuals were distinct. In contrast, all isolates obtained from mass-infection cases at a single institution had an identical genotype, suggesting that these cases were caused by a single E. histolytica strain. No significant correlation was found between genotypes and zymodemes or between genotypes and clinical presentations, e.g., colitis or liver abscess. Certain genotypes were observed with higher frequencies in male homosexuals or residents of institutions. These data indicate that genotyping of the E. histolytica isolates by using these four polymorphic loci could serve as a tool to fingerprint individual isolates. We propose that genotyping of ameba isolates should help to determine geographic origins of isolates and routes of transmission.

Original languageEnglish
Pages (from-to)4081-4090
Number of pages10
JournalJournal of Clinical Microbiology
Volume40
Issue number11
DOIs
Publication statusPublished - 2002 Nov 1
Externally publishedYes

Fingerprint

Entamoeba histolytica
Genetic Polymorphisms
Genotype
Chitinases
Nucleotides
Amebiasis
Polymerase Chain Reaction
Liver Abscess
Amoeba
Mentally Disabled Persons
Dermatoglyphics
Colitis
Sexual Minorities
DNA
Infection
Population
Genes
Proteins

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Remarkable genetic polymorphism among Entamoeba histolytica isolates from a limited geographic area. / Haghighi, Ali; Kobayashi, Seiki; Takeuchi, Tsutomu; Masuda, Gohta; Nozaki, Tomoyoshi.

In: Journal of Clinical Microbiology, Vol. 40, No. 11, 01.11.2002, p. 4081-4090.

Research output: Contribution to journalArticle

Haghighi, Ali ; Kobayashi, Seiki ; Takeuchi, Tsutomu ; Masuda, Gohta ; Nozaki, Tomoyoshi. / Remarkable genetic polymorphism among Entamoeba histolytica isolates from a limited geographic area. In: Journal of Clinical Microbiology. 2002 ; Vol. 40, No. 11. pp. 4081-4090.
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