Remarkable sequence similarity between the dinoflagellate-infecting marine girus and the terrestrial pathogen African swine fever virus

Hiroyuki Ogata, Kensuke Toyoda, Yuji Tomaru, Natsuko Nakayama, Yoko Shirai, Jean Michel Claverie, Keizo Nagasaki

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Heterocapsa circularisquama DNA virus (HcDNAV; previously designated as HcV) is a giant virus (girus) with a ∼356-kbp double-stranded DNA (dsDNA) genome. HcDNAV lytically infects the bivalve-killing marine dinoflagellate H. circularisquama, and currently represents the sole DNA virus isolated from dinoflagellates, one of the most abundant protists in marine ecosystems. Its morphological features, genome type, and host range previously suggested that HcDNAV might be a member of the family Phycodnaviridae of Nucleo-Cytoplasmic Large DNA Viruses (NCLDVs), though no supporting sequence data was available. NCLDVs currently include two families found in aquatic environments (Phycodnaviridae, Mimiviridae), one mostly infecting terrestrial animals (Poxviridae), another isolated from fish, amphibians and insects (Iridoviridae), and the last one (Asfarviridae) exclusively represented by the animal pathogen African swine fever virus (ASFV), the agent of a fatal hemorrhagic disease in domestic swine. In this study, we determined the complete sequence of the type B DNA polymerase (PolB) gene of HcDNAV. The viral PolB was transcribed at least from 6 h post inoculation (hpi), suggesting its crucial function for viral replication. Most unexpectedly, the HcDNAV PolB sequence was found to be closely related to the PolB sequence of ASFV. In addition, the amino acid sequence of HcDNAV PolB showed a rare amino acid substitution within a motif containing highly conserved motif: YSDTDS was found in HcDNAV PolB instead of YGDTDS in most dsDNA viruses. Together with the previous observation of ASFV-like sequences in the Sorcerer II Global Ocean Sampling metagenomic datasets, our results further reinforce the ideas that the terrestrial ASFV has its evolutionary origin in marine environments.

Original languageEnglish
Article number178
JournalVirology Journal
Volume6
DOIs
Publication statusPublished - 2009

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African Swine Fever Virus
Asfarviridae
Dinoflagellida
Phycodnaviridae
DNA Viruses
Viruses
Mimiviridae
Iridoviridae
Genome
Poxviridae
Metagenomics
Bivalvia
Host Specificity
Amphibians
Amino Acid Substitution
Oceans and Seas
Ecosystem
Insects
Amino Acid Sequence
Fishes

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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Remarkable sequence similarity between the dinoflagellate-infecting marine girus and the terrestrial pathogen African swine fever virus. / Ogata, Hiroyuki; Toyoda, Kensuke; Tomaru, Yuji; Nakayama, Natsuko; Shirai, Yoko; Claverie, Jean Michel; Nagasaki, Keizo.

In: Virology Journal, Vol. 6, 178, 2009.

Research output: Contribution to journalArticle

Ogata, Hiroyuki ; Toyoda, Kensuke ; Tomaru, Yuji ; Nakayama, Natsuko ; Shirai, Yoko ; Claverie, Jean Michel ; Nagasaki, Keizo. / Remarkable sequence similarity between the dinoflagellate-infecting marine girus and the terrestrial pathogen African swine fever virus. In: Virology Journal. 2009 ; Vol. 6.
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