Repeated 0.5-Gy γ irradiation attenuates experimental autoimmune encephalomyelitis with up-regulation of regulatory T cells and suppression of IL17 production

Mitsutoshi Tsukimoto, Hiroko Nakatsukasa, Keigo Sugawara, Keiichiro Yamashita, Shuji Kojima

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

We recently reported attenuation of autoimmune disease by low-dose γ irradiation (repeated doses of 0.5 Gy) in MRL-lpr/lpr mice. Here we studied the effect of low-dose γ irradiation on experimental autoimmune encephalomyelitis (EAE), which is a rodent model of multiple sclerosis. SJL/J mice were immunized with myelin basic protein for the induction of EAE and were exposed to 0.5 Gy γ rays once a week for 4 weeks. Radiation suppressed incidence and disease severity scores and delayed the onset of pathological changes. Pro-inflammatory cytokines (IFN-γ and IL6), autoantibody and CD8+ cytotoxic T cells are involved in the appearance of EAE. Radiation suppressed the increases in these cytokines and the autoantibody production and blocked the increase in the population of CD8+ cytotoxic T cells. Production of IL17 by Th17 cells and/or γδ T cells, which plays a crucial role in EAE, was also significantly suppressed by radiation. Furthermore, a significant increase in regulatory T cells, which are involved in suppression of autoimmune disease, was found in irradiated EAE mice. These data suggest that low-dose γ irradiation attenuates EAE through suppression of pro-inflammatory cytokines, reduction of cytotoxic T cells and induction of regulatory T cells.

Original languageEnglish
Pages (from-to)429-436
Number of pages8
JournalRadiation Research
Volume170
Issue number4
DOIs
Publication statusPublished - 2008 Oct 1
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Radiation
  • Radiology Nuclear Medicine and imaging

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