Repeated Chromosome Splitting Targeted to δ Sequences in Saccharomyces cerevisiae

Minetaka Sugiyama, Masafumi Nishizawa, Kyouhei Hayashi, Yoshinobu Kaneko, Kiichi Fukui, Akio Kobayashi, Satoshi Harashima

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We have previously developed a chromosome-splitting technique based on homologous recombination in Saccharomyces cerevisiae. To facilitate chromosome splitting at multiple sites, we focused on the δ sequences that are distributed in more than 200 copies throughout the yeast genome. We constructed a new chromosome-splitting vector harboring the YFLWdelta4 sequence and the hisG-URA3-hisG cassette, and transformed yeast cells with this vector. The karyotype analysis of transformants showed that chromosomes XIV, III, and IV, or other chromosomes are split. After the excision of the URA3 gene, the transformant with split chromosome IV was subsequently transformed with the same vector. Karyotype analysis revealed that further splitting occurred at chromosome X, the split chromosome IV, or other chromosomes. These results indicate that δ sequences are efficient target sites for repeated chromosome splitting at multiple sites with a single vector.

Original languageEnglish
Pages (from-to)397-400
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume96
Issue number4
DOIs
Publication statusPublished - 2003 Jan 1

Keywords

  • Chromosome engineering
  • Genome
  • Yeast
  • δ repeated sequence

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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    Sugiyama, M., Nishizawa, M., Hayashi, K., Kaneko, Y., Fukui, K., Kobayashi, A., & Harashima, S. (2003). Repeated Chromosome Splitting Targeted to δ Sequences in Saccharomyces cerevisiae. Journal of Bioscience and Bioengineering, 96(4), 397-400. https://doi.org/10.1263/jbb.96.397