Repeated Chromosome Splitting Targeted to δ Sequences in Saccharomyces cerevisiae

Minetaka Sugiyama, Masafumi Nishizawa, Kyouhei Hayashi, Yoshinobu Kaneko, Kiichi Fukui, Akio Kobayashi, Satoshi Harashima

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We have previously developed a chromosome-splitting technique based on homologous recombination in Saccharomyces cerevisiae. To facilitate chromosome splitting at multiple sites, we focused on the δ sequences that are distributed in more than 200 copies throughout the yeast genome. We constructed a new chromosome-splitting vector harboring the YFLWdelta4 sequence and the hisG-URA3-hisG cassette, and transformed yeast cells with this vector. The karyotype analysis of transformants showed that chromosomes XIV, III, and IV, or other chromosomes are split. After the excision of the URA3 gene, the transformant with split chromosome IV was subsequently transformed with the same vector. Karyotype analysis revealed that further splitting occurred at chromosome X, the split chromosome IV, or other chromosomes. These results indicate that δ sequences are efficient target sites for repeated chromosome splitting at multiple sites with a single vector.

Original languageEnglish
Pages (from-to)397-400
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume96
Issue number4
DOIs
Publication statusPublished - 2003

Fingerprint

Chromosomes
Yeast
Saccharomyces cerevisiae
Karyotype
Yeasts
Genes
Homologous Recombination
X Chromosome
Genome
Cells

Keywords

  • δ repeated sequence
  • Chromosome engineering
  • Genome
  • Yeast

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Sugiyama, M., Nishizawa, M., Hayashi, K., Kaneko, Y., Fukui, K., Kobayashi, A., & Harashima, S. (2003). Repeated Chromosome Splitting Targeted to δ Sequences in Saccharomyces cerevisiae. Journal of Bioscience and Bioengineering, 96(4), 397-400. https://doi.org/10.1263/jbb.96.397

Repeated Chromosome Splitting Targeted to δ Sequences in Saccharomyces cerevisiae. / Sugiyama, Minetaka; Nishizawa, Masafumi; Hayashi, Kyouhei; Kaneko, Yoshinobu; Fukui, Kiichi; Kobayashi, Akio; Harashima, Satoshi.

In: Journal of Bioscience and Bioengineering, Vol. 96, No. 4, 2003, p. 397-400.

Research output: Contribution to journalArticle

Sugiyama, M, Nishizawa, M, Hayashi, K, Kaneko, Y, Fukui, K, Kobayashi, A & Harashima, S 2003, 'Repeated Chromosome Splitting Targeted to δ Sequences in Saccharomyces cerevisiae', Journal of Bioscience and Bioengineering, vol. 96, no. 4, pp. 397-400. https://doi.org/10.1263/jbb.96.397
Sugiyama, Minetaka ; Nishizawa, Masafumi ; Hayashi, Kyouhei ; Kaneko, Yoshinobu ; Fukui, Kiichi ; Kobayashi, Akio ; Harashima, Satoshi. / Repeated Chromosome Splitting Targeted to δ Sequences in Saccharomyces cerevisiae. In: Journal of Bioscience and Bioengineering. 2003 ; Vol. 96, No. 4. pp. 397-400.
@article{7e1a2b9c56f3458f8d16aa64dfabf512,
title = "Repeated Chromosome Splitting Targeted to δ Sequences in Saccharomyces cerevisiae",
abstract = "We have previously developed a chromosome-splitting technique based on homologous recombination in Saccharomyces cerevisiae. To facilitate chromosome splitting at multiple sites, we focused on the δ sequences that are distributed in more than 200 copies throughout the yeast genome. We constructed a new chromosome-splitting vector harboring the YFLWdelta4 sequence and the hisG-URA3-hisG cassette, and transformed yeast cells with this vector. The karyotype analysis of transformants showed that chromosomes XIV, III, and IV, or other chromosomes are split. After the excision of the URA3 gene, the transformant with split chromosome IV was subsequently transformed with the same vector. Karyotype analysis revealed that further splitting occurred at chromosome X, the split chromosome IV, or other chromosomes. These results indicate that δ sequences are efficient target sites for repeated chromosome splitting at multiple sites with a single vector.",
keywords = "δ repeated sequence, Chromosome engineering, Genome, Yeast",
author = "Minetaka Sugiyama and Masafumi Nishizawa and Kyouhei Hayashi and Yoshinobu Kaneko and Kiichi Fukui and Akio Kobayashi and Satoshi Harashima",
year = "2003",
doi = "10.1263/jbb.96.397",
language = "English",
volume = "96",
pages = "397--400",
journal = "Journal of Bioscience and Bioengineering",
issn = "1389-1723",
publisher = "Elsevier",
number = "4",

}

TY - JOUR

T1 - Repeated Chromosome Splitting Targeted to δ Sequences in Saccharomyces cerevisiae

AU - Sugiyama, Minetaka

AU - Nishizawa, Masafumi

AU - Hayashi, Kyouhei

AU - Kaneko, Yoshinobu

AU - Fukui, Kiichi

AU - Kobayashi, Akio

AU - Harashima, Satoshi

PY - 2003

Y1 - 2003

N2 - We have previously developed a chromosome-splitting technique based on homologous recombination in Saccharomyces cerevisiae. To facilitate chromosome splitting at multiple sites, we focused on the δ sequences that are distributed in more than 200 copies throughout the yeast genome. We constructed a new chromosome-splitting vector harboring the YFLWdelta4 sequence and the hisG-URA3-hisG cassette, and transformed yeast cells with this vector. The karyotype analysis of transformants showed that chromosomes XIV, III, and IV, or other chromosomes are split. After the excision of the URA3 gene, the transformant with split chromosome IV was subsequently transformed with the same vector. Karyotype analysis revealed that further splitting occurred at chromosome X, the split chromosome IV, or other chromosomes. These results indicate that δ sequences are efficient target sites for repeated chromosome splitting at multiple sites with a single vector.

AB - We have previously developed a chromosome-splitting technique based on homologous recombination in Saccharomyces cerevisiae. To facilitate chromosome splitting at multiple sites, we focused on the δ sequences that are distributed in more than 200 copies throughout the yeast genome. We constructed a new chromosome-splitting vector harboring the YFLWdelta4 sequence and the hisG-URA3-hisG cassette, and transformed yeast cells with this vector. The karyotype analysis of transformants showed that chromosomes XIV, III, and IV, or other chromosomes are split. After the excision of the URA3 gene, the transformant with split chromosome IV was subsequently transformed with the same vector. Karyotype analysis revealed that further splitting occurred at chromosome X, the split chromosome IV, or other chromosomes. These results indicate that δ sequences are efficient target sites for repeated chromosome splitting at multiple sites with a single vector.

KW - δ repeated sequence

KW - Chromosome engineering

KW - Genome

KW - Yeast

UR - http://www.scopus.com/inward/record.url?scp=0345491336&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0345491336&partnerID=8YFLogxK

U2 - 10.1263/jbb.96.397

DO - 10.1263/jbb.96.397

M3 - Article

C2 - 16233544

AN - SCOPUS:0345491336

VL - 96

SP - 397

EP - 400

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

SN - 1389-1723

IS - 4

ER -