Repeated gamma irradiation attenuates collagen-induced arthritis via up-regulation of regulatory T cells but not by damaging lymphocytes directly

Hiroko Nakatsukasa, Mitsutoshi Tsukimoto, Akihiro Tokunaga, Shuji Kojima

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We recently reported that repeated 0.5-Gy irradiation attenuates the pathology of collagen-induced arthritis. In this study, to investigate the mechanism further, we focused on changes in Treg/Th17 cells and changes in the production of antibody against an external antigen in response to irradiation as well as on the radiosensitivity of Treg cells. DBA/1J mice were immunized with type II collagen to induce arthritis and exposed to low-dose rays (0.5 Gy/week for 5 weeks). Production of IL6 and IL17 as well as autoantibody was suppressed by irradiation in the early phase of collagen-induced arthritis. The percentage of Treg cells was significantly increased by irradiation at 4, 6 and 8 weeks after the immunization. We also investigated the effect of repeated radiation on the production of antibodies against an external antigen in ovalbumin-immunized BALB/c mice. We found that repeated 0.5-Gy irradiation enhanced antibody production, accompanied by an increase of the antibody-producing plasma cell population and increased Th2-type cytokine secretion. We also found that the radiosensitivity of Treg cells did not differ from that of other T cells. These results suggest that a major mechanism of attenuation of the pathology of collagen-induced arthritis by repeated 0.5-Gy irradiation is up-regulation of Treg cells concomitantly with suppression of IL6 and IL17 production.

Original languageEnglish
Pages (from-to)313-324
Number of pages12
JournalRadiation Research
Volume174
Issue number3
DOIs
Publication statusPublished - 2010 Jan 1
Externally publishedYes

Fingerprint

arthritis
Experimental Arthritis
lymphocytes
Regulatory T-Lymphocytes
collagens
Up-Regulation
Lymphocytes
antibodies
irradiation
Antibody Formation
Radiation Tolerance
cells
pathology
antigens
radiation tolerance
Interleukin-6
mice
Pathology
Th17 Cells
Antigens

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation
  • Medicine(all)

Cite this

Repeated gamma irradiation attenuates collagen-induced arthritis via up-regulation of regulatory T cells but not by damaging lymphocytes directly. / Nakatsukasa, Hiroko; Tsukimoto, Mitsutoshi; Tokunaga, Akihiro; Kojima, Shuji.

In: Radiation Research, Vol. 174, No. 3, 01.01.2010, p. 313-324.

Research output: Contribution to journalArticle

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