Repeated longitudinal in vivo imaging of neuro-glio-vascular unit at the peripheral boundary of ischemia in mouse cerebral cortex

K. Masamoto, Y. Tomita, H. Toriumi, I. Aoki, M. Unekawa, H. Takuwa, Y. Itoh, N. Suzuki, I. Kanno

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Understanding the cellular events evoked at the peripheral boundary of cerebral ischemia is critical for therapeutic outcome against the insult of cerebral ischemia. The present study reports a repeated longitudinal imaging for cellular-scale changes of neuro-glia-vascular unit at the boundary of cerebral ischemia in mouse cerebral cortex in vivo. Two-photon microscopy was used to trace the longitudinal changes of cortical microvasculature and astroglia following permanent middle cerebral artery occlusion (MCAO). We found that sulforhodamine 101 (SR101), a previously-known marker of astroglia, provide a bright signal in the vessels soon after the intraperitoneal injection, and that intensity was sufficient to detect the microvasculature up to a depth of 0.8. mm. After 5-8. h from the injection of SR101, cortical astroglia was also imaged up to a depth of 0.4. mm. After 1. day from MCAO, some microvessels showed a closure of the lumen space in the occluded MCA territory, leading to a restructuring of microvascular networks up to 7. days after MCAO. At the regions of the distorted microvasculature, an increase in the number of cells labeled with SR101 was detected, which was found as due to labeled neurons. Immunohistochemical results further showed that ischemia provokes neuronal uptake of SR101, which delineate a boundary between dying and surviving cells at the peripheral zone of ischemia in vivo. Finally, reproducibility of the MCAO model was evaluated with magnetic resonance imaging (MRI) in a different animal group, which showed the consistent infarct volume at the MCA territory over the subjects.

Original languageEnglish
Pages (from-to)190-200
Number of pages11
JournalNeuroscience
Volume212
DOIs
Publication statusPublished - 2012 Jun 14

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Microvessels
Cerebral Cortex
Middle Cerebral Artery Infarction
Blood Vessels
Ischemia
Brain Ischemia
Astrocytes
Intraperitoneal Injections
Photons
Neuroglia
Microscopy
Cell Count
Magnetic Resonance Imaging
Neurons
Injections
sulforhodamine 101
Therapeutics

Keywords

  • Cerebral microcirculation
  • Chronic cranial window
  • Fluorescent imaging
  • MRI
  • Sulforhodamine
  • Two-photon microscopy

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Repeated longitudinal in vivo imaging of neuro-glio-vascular unit at the peripheral boundary of ischemia in mouse cerebral cortex. / Masamoto, K.; Tomita, Y.; Toriumi, H.; Aoki, I.; Unekawa, M.; Takuwa, H.; Itoh, Y.; Suzuki, N.; Kanno, I.

In: Neuroscience, Vol. 212, 14.06.2012, p. 190-200.

Research output: Contribution to journalArticle

Masamoto, K, Tomita, Y, Toriumi, H, Aoki, I, Unekawa, M, Takuwa, H, Itoh, Y, Suzuki, N & Kanno, I 2012, 'Repeated longitudinal in vivo imaging of neuro-glio-vascular unit at the peripheral boundary of ischemia in mouse cerebral cortex', Neuroscience, vol. 212, pp. 190-200. https://doi.org/10.1016/j.neuroscience.2012.03.034
Masamoto, K. ; Tomita, Y. ; Toriumi, H. ; Aoki, I. ; Unekawa, M. ; Takuwa, H. ; Itoh, Y. ; Suzuki, N. ; Kanno, I. / Repeated longitudinal in vivo imaging of neuro-glio-vascular unit at the peripheral boundary of ischemia in mouse cerebral cortex. In: Neuroscience. 2012 ; Vol. 212. pp. 190-200.
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AU - Unekawa, M.

AU - Takuwa, H.

AU - Itoh, Y.

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AU - Kanno, I.

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AB - Understanding the cellular events evoked at the peripheral boundary of cerebral ischemia is critical for therapeutic outcome against the insult of cerebral ischemia. The present study reports a repeated longitudinal imaging for cellular-scale changes of neuro-glia-vascular unit at the boundary of cerebral ischemia in mouse cerebral cortex in vivo. Two-photon microscopy was used to trace the longitudinal changes of cortical microvasculature and astroglia following permanent middle cerebral artery occlusion (MCAO). We found that sulforhodamine 101 (SR101), a previously-known marker of astroglia, provide a bright signal in the vessels soon after the intraperitoneal injection, and that intensity was sufficient to detect the microvasculature up to a depth of 0.8. mm. After 5-8. h from the injection of SR101, cortical astroglia was also imaged up to a depth of 0.4. mm. After 1. day from MCAO, some microvessels showed a closure of the lumen space in the occluded MCA territory, leading to a restructuring of microvascular networks up to 7. days after MCAO. At the regions of the distorted microvasculature, an increase in the number of cells labeled with SR101 was detected, which was found as due to labeled neurons. Immunohistochemical results further showed that ischemia provokes neuronal uptake of SR101, which delineate a boundary between dying and surviving cells at the peripheral zone of ischemia in vivo. Finally, reproducibility of the MCAO model was evaluated with magnetic resonance imaging (MRI) in a different animal group, which showed the consistent infarct volume at the MCA territory over the subjects.

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